使用脉冲超连续谱激光对脂质进行多光谱光声显微镜成像。
Multispectral photoacoustic microscopy of lipids using a pulsed supercontinuum laser.
作者信息
Buma Takashi, Conley Nicole C, Choi Sang Won
机构信息
Department of Electrical, Computer, and Biomedical Engineering, Union College, Schenectady, NY 12308, USA.
Currently with the Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
出版信息
Biomed Opt Express. 2017 Dec 19;9(1):276-288. doi: 10.1364/BOE.9.000276. eCollection 2018 Jan 1.
Abstract
We demonstrate optical resolution photoacoustic microscopy (OR-PAM) of lipid-rich tissue between 1050-1714 nm using a pulsed supercontinuum laser based on a large-mode-area photonic crystal fiber. OR-PAM experiments of lipid-rich samples show the expected optical absorption peaks near 1210 and 1720 nm. These results show that pulsed supercontinuum lasers are promising for OR-PAM applications such as label-free histology of lipid-rich tissue and imaging small animal models of disease.
摘要
我们使用基于大模场面积光子晶体光纤的脉冲超连续谱激光器，展示了在1050 - 1714纳米之间对富含脂质组织的光学分辨率光声显微镜（OR-PAM）。富含脂质样本的OR-PAM实验显示，在1210和1720纳米附近出现了预期的光吸收峰。这些结果表明，脉冲超连续谱激光器在诸如富含脂质组织的无标记组织学以及疾病小动物模型成像等OR-PAM应用中具有广阔前景。
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