Department of Urology, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.
Department of Anesthesia, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.
Mol Med Rep. 2018 Mar;17(3):3853-3858. doi: 10.3892/mmr.2017.8318. Epub 2017 Dec 19.
Although alterations in microRNA (miRNA) expression have been previously investigated prostate cancer, the expression of miRNAs specifically in benign prostate hyperplasia (BPH) of the prostatic stroma remains to be fully elucidated. In the present study, miRNAs and gene expression profiles were investigated using microarray analysis and reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) in BPH tissue to clarify the associations between miRNA expression and target genes. Prostate tissue samples from five patients with BPH and five healthy men were analyzed using human Affymetrix miRNA and mRNA microarrays and differentially expressed miRNAs were validated using RT‑qPCR with 30 BPH and 5 healthy control samples. A total of 8 miRNAs, including miRNA (miR)‑96‑5p, miR‑1271‑5p, miR‑21‑3p, miR‑96‑5p, miR‑181a‑5p, miR‑143‑3p, miR‑4428 and miR‑106a‑5p were upregulated and 8 miRNAs (miR‑16‑5p, miR‑19b‑5p, miR‑940, miR‑25, miR‑486‑3p, miR‑30a‑3p, let‑7c and miR‑191) were downregulated. Additionally, miR‑96‑5p was demonstrated to have an inhibitory effect on the mRNA expression levels of the following genes: Mechanistic target of rapamycin (MTOR), RPTOR independent companion of MTOR complex 2, syntaxin 10, autophagy‑related protein 9A, zinc finger E‑box binding homeobox 1, caspase 2 and protein kinase c ε. Additionally, 16 differentially expressed miRNAs were identified using RT‑qPCR analysis. This preliminary study provides a solid basis for a further functional study to investigate the underlying regulatory mechanisms of BPH.
虽然 miRNA（miRNA）表达的改变已在前列腺癌中进行了研究，但 miRNA 在前列腺基质良性前列腺增生（BPH）中的特异性表达仍有待充分阐明。在本研究中，通过微阵列分析和逆转录定量聚合酶链反应（RT-qPCR）研究了 BPH 组织中的 miRNA 和基因表达谱，以阐明 miRNA 表达与靶基因之间的关联。使用人类 Affymetrix miRNA 和 mRNA 微阵列分析了来自 5 名 BPH 患者和 5 名健康男性的前列腺组织样本，并使用 RT-qPCR 对 30 个 BPH 和 5 个健康对照样本进行了验证。共鉴定出 8 个 miRNA，包括 miRNA（miR）-96-5p、miR-1271-5p、miR-21-3p、miR-96-5p、miR-181a-5p、miR-143-3p、miR-4428 和 miR-106a-5p 上调，8 个 miRNA（miR-16-5p、miR-19b-5p、miR-940、miR-25、miR-486-3p、miR-30a-3p、let-7c 和 miR-191）下调。此外，miR-96-5p 被证明对以下基因的 mRNA 表达水平具有抑制作用：雷帕霉素（mTOR）的机制靶标、mTOR 复合物 2 的 RPTOR 非依赖性伴侣、突触素 10、自噬相关蛋白 9A、锌指 E 框结合同源盒 1、半胱天冬酶 2 和蛋白激酶 c ε。此外，通过 RT-qPCR 分析鉴定了 16 个差异表达的 miRNA。这项初步研究为进一步的功能研究提供了坚实的基础，以研究 BPH 的潜在调节机制。
