Hannoversches Zentrum für Optische Technologien (HOT), Leibniz Universität Hannover, Hannover, Germany.
Klinik und Poliklinik für Dermatologie und Venerologie, Universitätsmedizin Rostock, Rostock, Germany.
J Biophotonics. 2018 Jun;11(6):e201700288. doi: 10.1002/jbio.201700288. Epub 2018 Feb 26.
A new multimodal system for rapid, noninvasive in vivo skin cancer screening is presented, combining optical coherence tomography (OCT) and optoacoustic (OA) modalities to provide precise tumor depth determination with a Raman spectroscopic modality capable of detecting the lesion type and, thus, providing diagnostic capability. Both OA and Raman setups use wide field skin illumination to ensure the compliance with maximum permissible exposure (MPE) requirements. The Raman signal is collected via the OCT scanning lens to maximize the signal-to-noise ratio of the measured signal while keeping radiation levels below MPE limits. OCT is used to optically determine the tumor thickness and for volumetric imaging whereas OA utilizes acoustic signals generated by optical absorption contrast for thickness determination at potentially higher penetration depths compared to OCT. Preliminary results of first clinical trials using our setup are presented. The measured lesion depth is in good agreement with histology results, while Raman measurements show distinctive differences between normal skin and melanocytic lesions, and, moreover, between different skin areas. In future, we will validate the setup presented for reliable detection of pathophysiological parameters, morphology and thickness of suspicious skin lesions.
提出了一种新的用于快速、无创体内皮肤癌筛查的多模态系统，结合光学相干断层扫描（OCT）和光声（OA）模态，以拉曼光谱模态提供精确的肿瘤深度确定，该模态能够检测病变类型，从而提供诊断能力。OA 和拉曼两种设置都使用宽场皮肤照明，以确保符合最大允许暴露（MPE）要求。拉曼信号通过 OCT 扫描透镜收集，以在保持辐射水平低于 MPE 限制的情况下最大化测量信号的信噪比。OCT 用于光学确定肿瘤厚度和体积成像，而 OA 利用光学吸收对比度产生的声信号用于在与 OCT 相比具有潜在更高穿透深度的情况下进行厚度确定。介绍了首次使用我们的设置进行的临床前试验的初步结果。测量的病变深度与组织学结果吻合良好，而拉曼测量显示正常皮肤和黑色素病变之间存在明显差异，此外，不同皮肤区域之间也存在差异。将来，我们将验证所提出的设置，以可靠地检测可疑皮肤病变的病理生理参数、形态和厚度。
