毛细管电泳系统（CAPILLARYS 2）检测血红蛋白变异体的精准度基于其迁移位置。
Precision of CAPILLARYS 2 for the Detection of Hemoglobin Variants Based on Their Migration Positions.
机构信息
Genetic Biochemistry Department, Henri Mondor Hospital, Creteil, France.
Division of Hematopathology, Mayo Clinic, Rochester, MN.
出版信息
Am J Clin Pathol. 2018 Jan 29;149(2):172-180. doi: 10.1093/ajcp/aqx148.
OBJECTIVES
In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument.
METHODS
The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants.
RESULTS
The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units.
CONCLUSION
In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.
目的
本报告评估了毛细管电泳（CE）仪器毛细管电泳（CE）迁移位置的实用性。
方法
选择常见的血红蛋白（Hb）变异体（Hb S、Hb C、Hb D-Punjab、Hb E、Hb Hope）来确定此 x 轴数字的精密度，并通过比较两个大型参考实验室对 81 种 Hb 变异体的结果来评估此数字的重现性。此外，还给出了总共 409 种 Hb 变异体的 CE 迁移位置。
结果
x 轴迁移位置显示出极好的内和间实验精度。对两个实验室所见 Hb 变异体的比较表明，83%的变异体的迁移位置差异在 1 个单位或以下。只有三种罕见的 Hb 变异体的差异超过 2 个单位。
结论
总之，CE 迁移位置是一个可重复的值，可以作为鉴定 Hb 变异体的辅助手段。
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