Millogo Missa, Soubeiga Serge Theophile, Bazie Bapio Valerie Jean Telesphore, Zohoncon Theodora Mahoukede, Ouattara Abdoul Karim, Yonli Albert Theophane, Simpore Jacques
Direction of Scientific and Technical Police, Ouagadougou, Burkina Faso.
Laboratory of Molecular Biology and Genetics (LABIOGENE) of University Joseph Ki-Zerbo, P.O. 03 BOX, 7021 Ouaga 03, Ouagadougou, Burkina Faso.
J Genet Eng Biotechnol. 2021 Aug 30;19(1):130. doi: 10.1186/s43141-021-00221-3.
In resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost. The limitations of these tests in cases of disputed paternity require the use of Short Tandem Repeats (STR) for their certification. This study aimed to determine the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays in Burkina Faso, West Africa.
Of the fourteen trios studied, the ABO-rhesus/hemoglobin electrophoresis analysis revealed ten probable inclusion cases, three exclusion cases, and one undetermined paternity. DNA STR analysis found five inclusions of paternity out of the ten probable inclusions with ABO-rhesus/hemoglobin electrophoresis assay versus nine exclusions of paternity.
This study showed that the implementation of the analysis of short tandem repeat is required to resolve increasing disputed filiation cases in Burkina Faso.
在资源有限的国家，ABO血型、人类白细胞抗原（HLA）、MNS血型系统、凯尔血型系统以及血红蛋白电泳因其成本低廉，是解决亲子关系纠纷的经典检测方法。这些检测方法在亲子关系有争议的案例中存在局限性，因此需要使用短串联重复序列（STR）进行鉴定。本研究旨在利用ABO血型-恒河猴血型/血红蛋白电泳和STR检测法确定西非布基纳法索儿童的生物学父亲。
在所研究的14个三联体中，ABO血型-恒河猴血型/血红蛋白电泳分析显示有10例可能的父子关系认定案例、3例排除案例和1例父子关系无法确定的案例。DNA STR分析在ABO血型-恒河猴血型/血红蛋白电泳检测法确定的10例可能的父子关系认定案例中发现了5例父子关系认定案例，同时发现了9例父子关系排除案例。
本研究表明，在布基纳法索，需要采用短串联重复序列分析来解决日益增多的亲子关系有争议的案例。
