Smith B A, Clark W R, McConnell H M
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5641-4. doi: 10.1073/pnas.76.11.5641.
Photobleaching in a two-dimensional periodic pattern has been used to characterize the motion of succinylconcanavalin A receptors on the surfaces of mouse fibroblasts. These receptors were found to exhibit anisotropic two-dimensional diffusion in adherent cells having parallel stress fibers. Diffusion coefficients of the order of 10(-11) cm2/sec and 10(-12) cm2/sec were measured for diffusive motions parallel and perpendicular to these fibers. The determination of the anisotropic motion used a two-dimensional Fourier analysis. A study was made of the effect of changes in cell shape on the photobleach pattern.
二维周期性模式下的光漂白已被用于表征小鼠成纤维细胞表面琥珀酰伴刀豆球蛋白A受体的运动。这些受体在具有平行应力纤维的贴壁细胞中表现出各向异性的二维扩散。对于平行和垂直于这些纤维的扩散运动，测量得到的扩散系数分别约为10(-11) cm2/秒和10(-12) cm2/秒。各向异性运动的测定采用二维傅里叶分析。研究了细胞形状变化对光漂白模式的影响。
