Spotiton：新特性和新应用。
Spotiton: New features and applications.
机构信息
The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, USA.
The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, USA; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
出版信息
J Struct Biol. 2018 May;202(2):161-169. doi: 10.1016/j.jsb.2018.01.002.
Abstract
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.
摘要
我们介绍了一种新型的用于冷冻电镜样品玻璃化的仪器 Spotiton 的更新版本，描述了其新特性和新应用。我们使用 Spotiton 制备了几个测试样本，这些样本可以使用常规的单颗粒分析方法重建到约 3Å 的分辨率，这表明该过程对样品完整性没有明显的有害影响。该系统现在在我们的实验室中常规且持续使用，并已成功用于各种样品的玻璃化。
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