水溶性磷光铱配合物的合成及其作为人血清白蛋白的开启型传感材料的应用。
Synthesis and application of a water-soluble phosphorescent iridium complex as turn-on sensing material for human serum albumin.
机构信息
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
出版信息
Dalton Trans. 2018 Feb 13;47(7):2330-2336. doi: 10.1039/c7dt04676f.
A novel water-soluble cyclometallated iridium complex [Ir(pq-COOH)FDS] (pq-COOH = 2-phenylquinoline-4-carboxylic acid, FDS = 3-(2-pyridyl)-5,6-bis(4-sulfophenyl)-1,2,4-triazine dianions) (abbreviated as Ir) was synthesized and its phosphorescent property was comprehensively studied. It was found that the complex exhibited strong phosphorescence, which peaked at 634 nm in neutral conditions (maximized at pH 8.0). Its phosphorescence decreased with an increase in acidity of the aqueous solution. At pH 2.0, the quenched phosphorescence could be resumed upon the addition of human serum albumin (HSA) because of the hydrophobic and electrostatic interactions between HSA and Ir. Based on this phenomenon, a "turn on" type phosphorescence probe was developed for the detection of HSA. Under optimal conditions, a wide calibration range of 1-280 nM was obtained with a limit of detection of 0.8 nM for HSA. The phosphorescence probe was successfully used for the determination of HSA in blood serum and urine samples.
一种新型水溶性金属铱配合物[Ir(pq-COOH)FDS]（pq-COOH=2-苯基喹啉-4-羧酸，FDS=3-(2-吡啶基)-5,6-双(4-磺基苯基)-1,2,4-三嗪二阴离子）（简称 Ir）被合成出来，并对其磷光性质进行了全面研究。结果发现，该配合物具有较强的磷光，在中性条件下（在 pH8.0 时最大）峰值位于 634nm。随着水溶液酸度的增加，其磷光强度逐渐降低。在 pH2.0 时，由于 HSA（人血清白蛋白）与 Ir 之间的疏水和静电相互作用，猝灭的磷光可以恢复。基于这一现象，开发了一种“开启”型磷光探针用于检测 HSA。在最佳条件下，该探针对 HSA 的检测范围为 1-280 nM，检测限为 0.8 nM。该磷光探针成功用于血清和尿液样品中 HSA 的测定。
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