College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China.
Dalton Trans. 2019 Jun 14;48(22):7728-7734. doi: 10.1039/c9dt01307e. Epub 2019 May 8.
The polarity of a cell is the feedback of a series of complex mechanisms that establish and maintain functionality of particular domains. Many cellular processes involved in the spatial arrangement and protein composition such as differentiation, localized membrane growth, activation of the immune response, directional cell migration, and vectorial transport of molecules across cell layers may lead to changes and development of polarity. In this work, a phosphorescent iridium complex was reported for sensitively probing environmental and cellular polarity. This probe exclusively targeted the endoplasmic reticulum (ER) and successfully in situ tracked polarity variations during ER stress in living cells. Importantly, the blood of diabetic mice in the presence of this probe appears to have distinguished phosphorescence compared with the blood of normal mice, indicating that the probe probably monitors blood polarity in diabetes.
细胞的极性是一系列复杂机制的反馈，这些机制建立并维持特定区域的功能。许多参与空间排列和蛋白质组成的细胞过程，如分化、局部膜生长、免疫反应激活、定向细胞迁移和分子在细胞层间的向量运输，都可能导致极性的变化和发展。在这项工作中，报道了一种磷光铱配合物，用于灵敏地探测环境和细胞极性。该探针专门针对内质网 (ER)，并成功地在活细胞中内质网应激过程中实时跟踪极性变化。重要的是，在存在该探针的糖尿病小鼠的血液中，磷光似乎与正常小鼠的血液有所区别，这表明该探针可能在糖尿病中监测血液极性。
