Dai Xixi, Liu Minggang, Pan Kangcheng, Yang Jinlong
Chongqing Academy of Animal Science, Rongchang, Chongqing, China.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
PLoS One. 2018 Jan 25;13(1):e0191627. doi: 10.1371/journal.pone.0191627. eCollection 2018.
Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determined by immunofluorescence microscopy. Mice immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores (p<0.01). In addition, oral immunization with recombinant spores was able to induce a significant level of protection in mice against lethal challenge with Salmonella serovar Typhimurium. These results suggest that B. subtilis spores have promising potential in the development of mucosal vaccines against Salmonella infections.
沙门氏菌病是全球主要的公共卫生问题。因此，迫切需要针对沙门氏菌感染的多样化控制策略。在这项工作中，我们评估了枯草芽孢杆菌（B. subtilis）孢子用于表达鸡白痢沙门氏菌主要保护性抗原OmpC的潜在用途。通过免疫荧光显微镜确定OmpC在孢子表面的表达。用表达OmpC抗原的重组孢子免疫的小鼠比用非重组孢子免疫的小鼠呈现出显著水平的OmpC特异性血清IgG和粘膜SIgA抗体（p<0.01）。此外，用重组孢子进行口服免疫能够在小鼠中诱导出显著水平的保护，使其免受鼠伤寒沙门氏菌的致死性攻击。这些结果表明，枯草芽孢杆菌孢子在开发针对沙门氏菌感染的粘膜疫苗方面具有广阔的潜力。
