a Department of Ophthalmology, Erasmus MC , University Medical Center , Rotterdam , The Netherlands.
b Department of Immunology, Laboratory Medical Immunology, Erasmus MC , University Medical Center , Rotterdam , The Netherlands.
Ocul Immunol Inflamm. 2019;27(3):418-423. doi: 10.1080/09273948.2017.1421671. Epub 2018 Jan 25.
: To analyze intraocular cytokine levels and cell profiles in patients with rubella virus-associated uveitis (RVU). : We collected intraocular fluid samples from patients with RVU ( = 10), uveitis of other causes ( = 27), and cataract ( = 22). Levels of 15 cytokines (IL-1β, IL-1ra, IL-2, IL-6, IL-6rα, IL-7, IL-8, IL-10, IL-17A, IL-23, TARC, MCP-1, TNF-α, PlGF, and VEGF) were measured using multiplex assay, and intraocular cell populations were determined by multiparameter flowcytometry. Clinical characteristics of RVU patients were collected and compared to laboratory outcomes. : RVU patients exhibited high intraocular levels of MCP-1, IL-6rα, and TARC, whilst patients with noninfectious uveitis were characterized by high levels of PlGF. Cataract patients showed high levels of IL-2 and IL-23. Intraocular cell population of RVU patients disclosed mainly T-cells and monocytes/macrophages and B-cells were scarcely detected. : RVU patients exhibit a cytokine profile distinct from noninfectious uveitis and cataract.
分析风疹病毒相关性葡萄膜炎（RVU）患者的眼内细胞因子水平和细胞特征。
我们收集了 10 例 RVU 患者、27 例其他病因的葡萄膜炎患者和 22 例白内障患者的眼内液样本。采用多重分析测定了 15 种细胞因子（IL-1β、IL-1ra、IL-2、IL-6、IL-6rα、IL-7、IL-8、IL-10、IL-17A、IL-23、TARC、MCP-1、TNF-α、PlGF 和 VEGF）的水平，并通过多参数流式细胞术测定了眼内细胞群。收集了 RVU 患者的临床特征并与实验室结果进行了比较。
RVU 患者表现出高浓度的眼内 MCP-1、IL-6rα 和 TARC，而非感染性葡萄膜炎患者的 PlGF 水平较高。白内障患者表现出高水平的 IL-2 和 IL-23。RVU 患者的眼内细胞群主要为 T 细胞和单核细胞/巨噬细胞，几乎检测不到 B 细胞。
RVU 患者表现出与非感染性葡萄膜炎和白内障不同的细胞因子特征。
