National University of Singapore Graduate School for Integrative Sciences and Engineering, Singapore, Singapore.
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Singapore, Singapore.
Sci Rep. 2018 Jan 25;8(1):1601. doi: 10.1038/s41598-018-20021-z.
L-ergothioneine (ET) is a diet-derived amino acid that accumulates at high concentrations in animals and humans. Numerous studies have highlighted its antioxidant abilities in vitro, and possible cytoprotective capabilities in vivo. We investigated the uptake and distribution of ET in various organs by a highly sensitive and specific liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) technique, both before and after oral administration of pure ET (35 and 70 mg/kg/day for 1, 7, and 28 days) to male C57BL6J mice. ET primarily concentrates in the liver and whole blood, and also in spleen, kidney, lung, heart, intestines, eye, and brain tissues. Strong correlations were found between ET and its putative metabolites - hercynine, ET-sulfonate (ET-SOH), and S-methyl ET. Hercynine accumulates in the brain after prolonged ET administration. This study demonstrates the uptake and distribution of ET and provides a foundation for future studies with ET to target oxidative damage in a range of tissues in human diseases.
L-ergothioneine (ET) 是一种饮食衍生的氨基酸，在动物和人类体内积累到很高的浓度。许多研究都强调了它在体外的抗氧化能力，以及在体内可能的细胞保护能力。我们使用高度敏感和特异的液相色谱串联质谱（LC-MS/MS）技术，在雄性 C57BL6J 小鼠口服纯 ET（35 和 70mg/kg/天，持续 1、7 和 28 天）前后，研究了 ET 在各种器官中的摄取和分布。ET 主要集中在肝脏和全血中，也集中在脾脏、肾脏、肺、心脏、肠道、眼睛和脑组织中。发现 ET 与其假定代谢物 - 赫琴宁、ET-硫酸盐（ET-SOH）和 S-甲基 ET 之间存在强烈的相关性。在长期给予 ET 后，赫琴宁在大脑中积累。这项研究表明了 ET 的摄取和分布，为未来在人类疾病中针对一系列组织的氧化损伤进行 ET 靶向治疗的研究提供了基础。
