Kang Jin Seok
Department of Biomedical Laboratory Science, Namseoul University, Cheonan, Korea.
Molecular Diagnostics Research Institute, Namseoul University, Cheonan, Korea.
Toxicol Res. 2018 Jan;34(1):1-6. doi: 10.5487/TR.2018.34.1.001. Epub 2018 Jan 15.
The purpose of this study was to detect cell death in the liver of mice treated with thioacetamide (TAA) using fluorescence bioimaging and compare this outcome with that using conventional histopathological examination. At 6 weeks of age, 24 mice were randomly divided into three groups: group 1 (G1), control group; group 2 (G2), fluorescence probe control group; group 3 (G3), TAA-treated group. G3 mice were treated with TAA. Twenty-two hours after TAA treatment, G2 and G3 mice were treated with Annexin-Vivo 750. Fluorescence bioimaging was performed by fluorescence molecular tomography at two hours after Annexin-Vivo 750 treatment, and fluorescence bioimaging of the liver was performed. Liver damage was validated by histopathological examination. bioimaging showed that the fluorescence intensity was increased in the right upper part of G3 mice compared with that in G2 mice, whereas G1 mice showed no signal. Additionally bioimaging showed that the fluorescence intensity was significantly increased in the livers of G3 mice compared with those in G1 or G2 mice ( < 0.05). Histopathological examination of the liver showed no cell death in G1 and G2 mice. However, in G3 mice, there was destruction of hepatocytes and increased cell death. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining confirmed many cell death features in the liver of G3 mice, whereas no pathological findings were observed in the liver of G1 and G2 mice. Taken together, fluorescence bioimaging in this study showed the detection of cell death and made it possible to quantify the level of cell death in male mice. The outcome was correlated with conventional biomedical examination. As it was difficult to differentiate histological location by fluorescent bioimaging, it is necessary to develop specific fluorescent dyes for monitoring hepatic disease progression and to exploit new bioimaging techniques without dye-labeling.
本研究的目的是使用荧光生物成像检测硫代乙酰胺（TAA）处理的小鼠肝脏中的细胞死亡，并将此结果与使用传统组织病理学检查的结果进行比较。6周龄时，将24只小鼠随机分为三组：第1组（G1），对照组；第2组（G2），荧光探针对照组；第3组（G3），TAA处理组。G3组小鼠用TAA处理。TAA处理22小时后，G2和G3组小鼠用膜联蛋白-Vivo 750处理。在膜联蛋白-Vivo 750处理后两小时通过荧光分子断层扫描进行荧光生物成像，并对肝脏进行荧光生物成像。通过组织病理学检查验证肝损伤。生物成像显示，与G2组小鼠相比，G3组小鼠右上部分的荧光强度增加，而G1组小鼠无信号。此外，生物成像显示，与G1或G2组小鼠相比，G3组小鼠肝脏中的荧光强度显著增加（<0.05）。肝脏的组织病理学检查显示G1和G2组小鼠无细胞死亡。然而，在G3组小鼠中，肝细胞有破坏且细胞死亡增加。末端脱氧核苷酸转移酶dUTP缺口末端标记染色证实G3组小鼠肝脏中有许多细胞死亡特征，而在G1和G2组小鼠肝脏中未观察到病理结果。综上所述，本研究中的荧光生物成像显示了细胞死亡的检测，并能够量化雄性小鼠的细胞死亡水平。该结果与传统生物医学检查相关。由于通过荧光生物成像难以区分组织学位置，因此有必要开发用于监测肝脏疾病进展的特异性荧光染料，并开发无需染料标记的新生物成像技术。
