Her G R, Watson J T
Biomed Environ Mass Spectrom. 1986 Feb;13(2):57-63. doi: 10.1002/bms.1200130203.
A procedure which can be used to increase the electron affinity of corticosteroids for purposes of quantitative analysis is proposed. Ten important corticosteroids are chemically oxidized to 1,4-androstadien-3,11,17-trione or 4-androsten-3,6,17-trione structural steroid analogs to enhance their electrophilicity prior to detection by electron capture negative chemical ionization mass spectrometry. The choice of oxidation reagent, a preliminary assessment of the structure-response relationship of the oxidation products, the optimization of negative ion formation as a function of source temperature and the basis for distinguishing the isomers dexamethasone and betamethasone are addressed.
提出了一种可用于提高皮质类固醇电子亲和力以进行定量分析的方法。在通过电子捕获负化学电离质谱法检测之前，将十种重要的皮质类固醇化学氧化为1,4 - 雄甾二烯 - 3,11,17 - 三酮或4 - 雄烯 - 3,6,17 - 三酮结构的类固醇类似物，以增强其亲电性。讨论了氧化试剂的选择、氧化产物结构 - 响应关系的初步评估、作为源温度函数的负离子形成优化以及区分地塞米松和倍他米松异构体的依据。
