Frese Cornelia, Wolff Diana, Krüger Tanja, Staehle Hans J, Lux Christopher J, Erber Ralf
Department of Conservative Dentistry, University Hospital Heidelberg.
Department of Orthodontics and Dentofacial Orthopaedics, University Hospital Heidelberg.
J Oral Sci. 2018 Mar 24;60(1):89-96. doi: 10.2334/josnusd.16-0827. Epub 2018 Jan 29.
Placement of composite resin restorations in deep subgingival cavities can damage surrounding soft tissues. In addition, commonly used resin-based composites (RBCs) might interfere with wound healing and periodontal health. To clarify cellular interactions with RBCs, we used an MTT assay to investigate adhesion of primary human gingival fibroblasts and human osteoblasts (hFOB 1.19) on five RBC materials with and without surface modifications (alumina blasting with 50- or 110-μm AlO). In addition, high-performance liquid chromatography (HPLC) was used to determine release of resin monomers from RBCs after 1 h, 1 day, and 7 days. As compared with tissue culture plastics (the control), cellular adhesion was significantly lower (P < 0.001) for human gingival fibroblasts and osteoblasts. Only minor, nonsignificant differences between individual RBCs were identified. HPLC analyses identified the release of three bifunctional methacrylates bisphenol A glycerolate dimethacrylate, triethylene glycol dimethacrylate, and diurethane dimethacrylate from RBCs and showed that monomer release increased between 1 h and 1 day but remained low. The present findings suggest that surface adhesion in the subgingival area is limited for the tested RBCs. Although residual monomer release was low for all tested RBCs, it might be sufficient to adversely affect cell adhesion.
在深龈下龋洞中放置复合树脂修复体可能会损伤周围软组织。此外，常用的树脂基复合材料（RBCs）可能会干扰伤口愈合和牙周健康。为了阐明细胞与RBCs的相互作用，我们使用MTT法研究了原代人牙龈成纤维细胞和人成骨细胞（hFOB 1.19）在五种经过和未经过表面改性（用50-或110-μm AlO进行氧化铝喷砂处理）的RBC材料上的粘附情况。此外，还使用高效液相色谱法（HPLC）测定了RBCs在1小时、1天和7天后树脂单体的释放情况。与组织培养塑料（对照）相比，人牙龈成纤维细胞和成骨细胞的细胞粘附显著降低（P < 0.001）。在各个RBCs之间仅发现了微小的、无统计学意义的差异。HPLC分析确定了RBCs释放出三种双官能甲基丙烯酸酯双酚A甘油酸酯二甲基丙烯酸酯、三甘醇二甲基丙烯酸酯和二氨基甲酸酯二甲基丙烯酸酯，并表明单体释放在1小时到1天之间增加，但仍然很低。目前的研究结果表明，对于所测试的RBCs，龈下区域的表面粘附是有限的。尽管所有测试的RBCs的残留单体释放量都很低，但可能足以对细胞粘附产生不利影响。
