Khojah Maysaa Z, Youssef Abdel-Rahman, Alsahafi Elham N
Preventive Dentistry Department, Orthodontic Division, Faculty of Dental Medicine, Umm Al-Qura University, Makkah, Saudi Arabia.
Department of Basic and Clinical Oral Sciences, Division of Basic Medical Sciences, Faculty of Dental Medicine, Umm Al-Qura University, Makkah, Saudi Arabia.
J Orthod Sci. 2025 Mar 25;14:3. doi: 10.4103/jos.jos_67_24. eCollection 2025.
Composite resins and bonding agents are indispensable in orthodontic practice, necessitating a thorough understanding of their cytotoxic effects, particularly when expired. This study aimed to evaluate the influence of expired composite and bonding materials on human gingival fibroblasts (hGFs).
Both expired and nonexpired composite resins and bonding agents were tested on hGFs using direct exposure methods. Viability assays, morphological evaluations, and wound healing assays were conducted at 24 and 72 hours post exposure.
Exposure to both expired and nonexpired materials led to significant reductions in hGF viability and alterations in morphology. Wound healing assays demonstrated impaired migratory abilities of hGFs following exposure to these materials.
The study highlights the importance of prudent material selection, handling, and monitoring in orthodontic practice to mitigate adverse effects on gingival tissues. Proper management of expired materials is crucial for cost-effectiveness, waste reduction, and patient safety. Further research into the long-term impacts of expired materials on gingival and periodontal health is imperative to ensure clinical treatment safety and efficacy. This investigation provides valuable insights into the biocompatibility of orthodontic bonding materials and emphasizes the necessity for continued vigilance in their usage to uphold patient welfare and treatment quality.
复合树脂和粘结剂在正畸治疗中不可或缺，因此有必要深入了解它们的细胞毒性作用，尤其是过期时的情况。本研究旨在评估过期的复合树脂和粘结材料对人牙龈成纤维细胞（hGFs）的影响。
使用直接暴露法，对过期和未过期的复合树脂及粘结剂在hGFs上进行测试。在暴露后24小时和72小时进行活力测定、形态学评估和伤口愈合测定。
暴露于过期和未过期材料均导致hGF活力显著降低和形态改变。伤口愈合测定表明，暴露于这些材料后hGF的迁移能力受损。
该研究强调了在正畸治疗中谨慎选择、处理和监测材料以减轻对牙龈组织不良影响的重要性。妥善管理过期材料对于成本效益、减少浪费和患者安全至关重要。进一步研究过期材料对牙龈和牙周健康的长期影响对于确保临床治疗的安全性和有效性势在必行。本研究为正畸粘结材料的生物相容性提供了有价值的见解，并强调了在其使用过程中持续保持警惕以维护患者福利和治疗质量的必要性。
