Flottes Yohann, Dursun Elisabeth
URP 4462, Innovative Dental Materials and Interfaces Research Unit, Paris Cité University, Montrouge, France.
Henri Mondor Hospital, AP-HP, Créteil, France.
Proteomics Clin Appl. 2025 Jan;19(1):e202400049. doi: 10.1002/prca.202400049. Epub 2024 Oct 18.
To investigate the potential effects of products released by a resin composite on the proteome of human gingival fibroblasts.
Fifteen resin composite cylinders of a Bis-GMA-based resin composite (Tetric EvoCeram, Ivoclar) were made and placed in a culture medium for 24 h. Then, 30 mL of this medium was placed for 72 h in contact with human gingival fibroblasts and a second control group consisted of cells placed in culture medium only. Afterward, cells were collected, washed, and their proteins extracted. Three two-dimensional electrophoresis were performed per condition. Image analysis of the gels was carried out to highlight the differential protein spots. These spots were then analyzed by an ESI/qTOF mass spectrometer. Finally, specific databases provided protein identification, their interactions, and the pathways where they are implicated.
Delta2D software allowed the detection of 21 spots of different proteins. The MASCOT identified 28 proteins. Five proteins from four spots were upregulated, 23 proteins from 17 spots were downregulated. The UniProt database showed that all these proteins were involved in cellular architecture, structural modifications and quality control of proteins, cellular homeostasis, and metabolic pathways. The STRING database revealed the interactions between the regulated proteins. The GO enrichment analysis showed that 19 pathways were affected.
The products released from the resin composite tested led to changes in the fibroblast proteome. Under the conditions of this study, resin composite released products can cause early adverse effects on cells, but without complete inhibition of their cellular functions.
研究树脂复合材料释放的产物对人牙龈成纤维细胞蛋白质组的潜在影响。
制作15个基于双酚A-甲基丙烯酸缩水甘油酯（Bis-GMA）的树脂复合材料（Tetric EvoCeram，义获嘉公司）圆柱体，并将其置于培养基中24小时。然后，取30mL该培养基与人类牙龈成纤维细胞接触72小时，另一个对照组仅由置于培养基中的细胞组成。之后，收集细胞，洗涤并提取其蛋白质。每种条件下进行三次二维电泳。对凝胶进行图像分析以突出差异蛋白点。然后通过电喷雾电离/四极杆飞行时间（ESI/qTOF）质谱仪分析这些点。最后，特定数据库提供蛋白质鉴定、它们的相互作用以及它们所涉及的途径。
Delta2D软件检测到21个不同蛋白质的斑点。MASCOT鉴定出28种蛋白质。来自4个斑点的5种蛋白质上调，来自17个斑点的23种蛋白质下调。UniProt数据库显示所有这些蛋白质都参与细胞结构、蛋白质的结构修饰和质量控制、细胞内稳态以及代谢途径。STRING数据库揭示了受调控蛋白质之间的相互作用。基因本体（GO）富集分析表明19条途径受到影响。
所测试的树脂复合材料释放的产物导致成纤维细胞蛋白质组发生变化。在本研究条件下，树脂复合材料释放的产物可对细胞产生早期不良影响，但不会完全抑制其细胞功能。
