Department of Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
Dis Markers. 2017;2017:7439698. doi: 10.1155/2017/7439698. Epub 2017 Nov 19.
Long noncoding RNAs (lncRNAs) have been reported to play vital roles in non-small-cell lung cancer (NSCLC). Recently, long noncoding RNA Linc00152 has been reported to play important roles in various cancers. In this study, our aim was to investigate its expression pattern and clinical significance and further evaluate its diagnostic value for NSCLC.
The levels of Linc00152 were detected in NSCLC tissues and plasma samples by quantitative real-time PCR (qRT-PCR). Receiver operating characteristic (ROC) curves were depicted to evaluate the diagnostic value.
We found that Linc00152 levels were upregulated in both NSCLC tissues and plasma samples. Plasma Linc00152 levels were significantly lower in postoperative samples than in preoperative samples. Besides, high Linc00152 expression was significantly correlated with tumor size ( = 0.293, = 0.005) and tumor stage ( = 0.324, = 0.011). The ROC curves indicated that plasma Linc00152 has high diagnostic accuracy for NSCLC, and the area under curve (AUC) for NSCLC versus healthy was 0.816 (95% CI: 0.757-0.875). Moreover, we found that the combination of Linc00152 and CEA could provide a more powerful diagnosis efficiency than Linc00152 or CEA alone (AUC = 0.881, 95% CI: 0.836-0.926).
Plasma Linc00152 could serve as a promising biomarker for diagnosing and monitoring NSCLC.
长链非编码 RNA（lncRNA）已被报道在非小细胞肺癌（NSCLC）中发挥重要作用。最近，长链非编码 RNA Linc00152 已被报道在各种癌症中发挥重要作用。在本研究中，我们旨在研究其表达模式和临床意义，并进一步评估其对 NSCLC 的诊断价值。
通过实时定量 PCR（qRT-PCR）检测 NSCLC 组织和血浆样本中的 Linc00152 水平。绘制受试者工作特征（ROC）曲线评估诊断价值。
我们发现 Linc00152 在 NSCLC 组织和血浆样本中均上调。术后样本血浆 Linc00152 水平明显低于术前样本。此外，高 Linc00152 表达与肿瘤大小（ = 0.293， = 0.005）和肿瘤分期（ = 0.324， = 0.011）显著相关。ROC 曲线表明，血浆 Linc00152 对 NSCLC 具有较高的诊断准确性，NSCLC 与健康对照的 AUC 为 0.816（95%CI：0.757-0.875）。此外，我们发现 Linc00152 与 CEA 的联合检测比单独检测 Linc00152 或 CEA 具有更高的诊断效率（AUC=0.881，95%CI：0.836-0.926）。
血浆 Linc00152 可作为诊断和监测 NSCLC 的有前途的生物标志物。
