Sah Sunil Kumar, González Joaquin V, Shrestha Sadina, Adhikari Anurag, Manandhar Krishna Das, Yadav Shyam Babu, Stein David A, Gupta Birendra Prasad, Picconi María Alejandra
1B. P. Koirala Memorial Cancer Hospital, Bharatpur, Chitwan Nepal.
2Oncogenic Viruses Laboratory, National Institute of Infectious Diseases-ANLIS "Dr. Malbrán", Av. Velez Sarsfield 563, C1282AFF Buenos Aires, Argentina.
Infect Agent Cancer. 2018 Jan 17;13:4. doi: 10.1186/s13027-018-0176-7. eCollection 2018.
Cervical cancer (CC) is the leading cause of morbidity and mortality from cancer in Nepalese women. Nearly all cases of CC are caused by infection with certain genotypes of human papillomavirus (HPV). Data on HPV genotype distribution in Nepalese CC patients is sparse. We aimed to determine the distribution of HPV genotypes in biopsies of CC tissue from Nepalese women.
This study examined 248 archived paraffin-embedded tissue specimens from CC cases from patients of B.P. Koirala Memorial Cancer Hospital, Bharatpur, Chitwan, Nepal. DNA was extracted from the biopsies and HPV detection performed by PCR. HPV genotyping was then carried out by a reverse line hybridization technique capable of identifying 36 distinct HPV genotypes.
Most of the samples were from tumors that had been designated by hospital pathologists as squamous cell carcinoma (77.6%). 165 of the 248 samples contained DNA of sufficient quality for rigorous PCR testing. All the analyzable specimens were positive for HPV. The most common HPV genotypes, in decreasing order of frequency were 16, 18, 45, 33, 52, 56 and 31; most were found as single infections (94.5%). Together, HPV types 16, 18, and 45 were found in 92% of the tumor samples.
This study strengthens the knowledge-base of HPV genotype distribution in CC cases in Nepal. Hopefully, this information will be useful to the medical community and public health policy-makers in generating improved HPV-surveillance, -prevention and -treatment strategies in Nepal.
宫颈癌（CC）是尼泊尔女性癌症发病和死亡的主要原因。几乎所有宫颈癌病例都是由某些基因型的人乳头瘤病毒（HPV）感染引起的。关于尼泊尔宫颈癌患者中HPV基因型分布的数据很少。我们旨在确定尼泊尔女性宫颈癌组织活检中HPV基因型的分布情况。
本研究检测了来自尼泊尔奇旺巴拉特普尔B.P.柯伊拉腊纪念癌症医院患者的248份存档石蜡包埋组织标本。从活检组织中提取DNA，并通过PCR进行HPV检测。然后采用能够识别36种不同HPV基因型的反向线杂交技术进行HPV基因分型。
大多数样本来自医院病理学家诊断为鳞状细胞癌的肿瘤（77.6%）。248份样本中有165份含有质量足以进行严格PCR检测的DNA。所有可分析的标本HPV检测均为阳性。最常见的HPV基因型按频率从高到低依次为16、18、45、33、52、56和31；大多数为单一感染（94.5%）。HPV 16、18和45型共出现在92%的肿瘤样本中。
本研究加强了尼泊尔宫颈癌病例中HPV基因型分布的知识库。希望这些信息将有助于医学界和公共卫生政策制定者在尼泊尔制定更好的HPV监测、预防和治疗策略。
