Leyendecker Junior Alessander, Gomes Pinheiro Carla Cristina, Lazzaretti Fernandes Tiago, Franco Bueno Daniela
Hospital Sírio Libanês, São Paulo, Brazil.
J Tissue Eng. 2018 Jan 17;9:2041731417752766. doi: 10.1177/2041731417752766. eCollection 2018 Jan-Dec.
Dental pulp represents a promising and easily accessible source of mesenchymal stem cells for clinical applications. Many studies have investigated the use of human dental pulp stem cells and stem cells isolated from the dental pulp of human exfoliated deciduous teeth for bone tissue engineering in vivo. However, the type of scaffold used to support the proliferation and differentiation of dental stem cells, the animal model, the type of bone defect created, and the methods for evaluation of results were extremely heterogeneous among these studies conducted. With this issue in mind, the main objective of this study is to present and summarize, through a systematic review of the literature, in vivo studies in which the efficacy of human dental pulp stem cells and stem cells from human exfoliated deciduous teeth (SHED) for bone regeneration was evaluated. The article search was conducted in PubMed/MEDLINE and Web of Science databases. Original research articles assessing potential of human dental pulp stem cells and SHED for in vivo bone tissue engineering, published from 1984 to November 2017, were selected and evaluated in this review according to the following eligibility criteria: published in English, assessing dental stem cells of human origin and evaluating in vivo bone tissue formation in animal models or in humans. From the initial 1576 potentially relevant articles identified, 128 were excluded due to the fact that they were duplicates and 1392 were considered ineligible as they did not meet the inclusion criteria. As a result, 56 articles remained and were fully analyzed in this systematic review. The results obtained in this systematic review open new avenues to perform bone tissue engineering for patients with bone defects and emphasize the importance of using human dental pulp stem cells and SHED to repair actual bone defects in an appropriate animal model.
牙髓是一种很有前景且易于获取的间充质干细胞来源，可用于临床应用。许多研究已经探讨了人类牙髓干细胞以及从人类脱落乳牙牙髓中分离出的干细胞在体内骨组织工程中的应用。然而，在这些已开展的研究中，用于支持牙髓干细胞增殖和分化的支架类型、动物模型、所制造的骨缺损类型以及结果评估方法极为不同。考虑到这一问题，本研究的主要目的是通过对文献的系统综述，呈现并总结那些评估人类牙髓干细胞和人类脱落乳牙干细胞（SHED）骨再生功效的体内研究。文章检索在PubMed/MEDLINE和Web of Science数据库中进行。本综述根据以下纳入标准，选取并评估了1984年至2017年11月发表的、评估人类牙髓干细胞和SHED在体内骨组织工程中潜力的原创性研究文章：以英文发表、评估人类来源的牙髓干细胞以及评估动物模型或人类体内的骨组织形成情况。从最初识别出的1576篇潜在相关文章中，128篇因重复而被排除，1392篇因不符合纳入标准而被认为不合格。结果，56篇文章留存并在本系统综述中进行了全面分析。本系统综述所获得的结果为骨缺损患者开展骨组织工程开辟了新途径，并强调了在合适的动物模型中使用人类牙髓干细胞和SHED修复实际骨缺损的重要性。
