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多类生物活性成分的定量测定用于中国十种、四种和一种物种的质量评估。

Quantitative determination of multi-class bioactive constituents for quality assessment of ten , four and one species in China.

作者信息

Wu Yan-Bin, Peng Meng-Chao, Zhang Chao, Wu Jian-Guo, Ye Bing-Zhu, Yi Jun, Wu Jin-Zhong, Zheng Cheng-Jian

机构信息

School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.

Department of Pharmacognosy, School of Pharmacy, Naval Medical University, Shanghai 200433, China.

出版信息

Chin Herb Med. 2020 Sep 4;12(4):430-439. doi: 10.1016/j.chmed.2020.07.002. eCollection 2020 Oct.

DOI:10.1016/j.chmed.2020.07.002
PMID:36120169
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9476377/
Abstract

OBJECTIVE

To establish multi-class bioactive constituents' determination of ten , four and one species, and provide reference for the improvement of their quality control.

METHODS

HPLC-ELSD and phenol-sulphuric acid methods were used for the quantitative determination of lactone glycosides (kinsenoside and its diastereoisomer, goodyeroside A) and polysaccharides, respectively, while an efficient iHPLC-MS/MS method was established for rapid determination of other minor constituents in ten species and five related species.

RESULTS

The contents of kinsenoside, goodyeroside A, polysaccharides and flavonoids varied notably almost in all tested samples, including both wild plants and tissue cultures. In particular, kinsenoside was the major lactone glycoside in , , , , and , whereas goodyeroside A was the predominant constituent in , , , , , , , and .

CONCLUSION

Our present study suggested that , , , , and species cannot be used as alternatives for . , and goodyeroside A may be reasonably used as a diagnostic marker for distinguishing from , , and , and species The established method thus could be potentially used for the quality evaluation and control of and some related species.

摘要

目的

建立10种、4种和1种植物中多类生物活性成分的测定方法，为提高其质量控制提供参考。

方法

采用高效液相色谱 - 蒸发光散射检测器（HPLC - ELSD）法和苯酚 - 硫酸法分别定量测定内酯糖苷（人参皂苷和其非对映异构体、球果假沙晶兰苷A）和多糖，同时建立了高效离子对液相色谱 - 串联质谱（iHPLC - MS/MS）法快速测定10种植物和5种相关植物中的其他微量成分。

结果

几乎在所有测试样品中，包括野生植物和组织培养物，人参皂苷、球果假沙晶兰苷A、多糖和黄酮类化合物的含量都有显著差异。特别是，人参皂苷是[植物名称1]、[植物名称2]、[植物名称3]、[植物名称4]、[植物名称5]和[植物名称6]中的主要内酯糖苷，而球果假沙晶兰苷A是[植物名称7]、[植物名称8]、[植物名称9]、[植物名称10]、[植物名称11]、[植物名称12]、[植物名称13]和[植物名称14]中的主要成分。

结论

我们目前的研究表明，[植物名称1]、[植物名称2]、[植物名称3]、[植物名称4]、[植物名称5]和[植物名称6]不能用作[目标植物名称]的替代品。人参皂苷和球果假沙晶兰苷A可合理用作区分[目标植物名称]与[植物名称7]、[植物名称8]、[植物名称9]、[植物名称10]、[植物名称11]、[植物名称12]、[植物名称13]和[植物名称14]的诊断标志物。因此，所建立的方法可潜在地用于[目标植物名称]和一些相关植物的质量评估和控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/e7f3b1bf8dee/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/2eb6f3f4be50/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/87b9d4668cb3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/3c435915c6ac/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/2549178e0e29/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/6b268d9d8bc8/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/e7f3b1bf8dee/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/2eb6f3f4be50/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/87b9d4668cb3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/3c435915c6ac/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/2549178e0e29/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/6b268d9d8bc8/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0ab/9476377/e7f3b1bf8dee/gr6.jpg

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