鼠伤寒沙门氏菌中Chi重组热点的活性
Activity of Chi recombinational hotspots in Salmonella typhimurium.
作者信息
Smith G R, Roberts C M, Schultz D W
出版信息
Genetics. 1986 Mar;112(3):429-39. doi: 10.1093/genetics/112.3.429.
Abstract
Chi sites have previously been shown to stimulate homologous recombination by the Escherichia coli RecBC pathway. To test the activity of Chi in another organism, bacteriophage lambda crosses were carried out in Salmonella typhimurium strains bearing the E. coli lambda receptor protein. Chi is active in these crosses in S. typhimurium, but is less active than in the same crosses carried out in E. coli. The lower Chi activity in S. typhimurium appears to be intrinsic to the S. typhimurium RecBC enzyme, since the Chi activity in E. coli-S. typhimurium hybrids depends on the species of origin of their RecBC enzyme. For these studies we constructed and F' factor and a pBR322-derived plasmid carrying the thyA+ recC+ recB+ argA+ region of the S. typhimurium chromosome.
摘要
此前已表明，χ位点可通过大肠杆菌RecBC途径刺激同源重组。为了测试χ在另一种生物体中的活性，在携带大肠杆菌λ受体蛋白的鼠伤寒沙门氏菌菌株中进行了噬菌体λ杂交。χ在鼠伤寒沙门氏菌的这些杂交中具有活性，但比在大肠杆菌中进行的相同杂交活性低。鼠伤寒沙门氏菌中较低的χ活性似乎是鼠伤寒沙门氏菌RecBC酶所固有的，因为大肠杆菌-鼠伤寒沙门氏菌杂种中的χ活性取决于其RecBC酶的来源物种。为了进行这些研究，我们构建了一个F'因子和一个携带鼠伤寒沙门氏菌染色体thyA+ recC+ recB+ argA+区域的pBR322衍生质粒。
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