类Chi序列的重组热点活性。

Recombinational hotspot activity of Chi-like sequences.

作者信息

Cheng K C, Smith G R

出版信息

J Mol Biol. 1984 Dec 5;180(2):371-7. doi: 10.1016/s0022-2836(84)80009-1.

Abstract

Chi sites, consisting of the nucleotide octamer 5' G-C-T-G-G-T-G-G 3', stimulate coliphage lambda recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage lambda crosses, three of four Chi-like sequences tested, namely 5' A-C-T-G-G-T-G-G 3', 5' G-T-T-G-G-T-G-G 3' and 5' G-C-T-A-G-T-G-G 3', had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of lambda lacking Chi sites.

摘要

由核苷酸八聚体5'-G-C-T-G-G-T-G-G-3'组成的Chi位点可刺激由大肠杆菌RecBC重组途径介导的λ噬菌体重组。在一项使用λ噬菌体杂交的灵敏遗传检测中，所测试的四个类Chi序列中的三个，即5'-A-C-T-G-G-T-G-G-3'、5'-G-T-T-G-G-T-G-G-3'和5'-G-C-T-A-G-T-G-G-3'，分别具有约6%、11%和38%的完整Chi活性。我们得出结论，某些类Chi序列表现出一系列重组热点活性，并且可能是缺乏Chi位点的λ噬菌体RecBC介导的普遍重组的原因。