拓展基质辅助激光解吸电离飞行时间质谱的极限：超越真菌物种鉴定
Pushing the Limits of MALDI-TOF Mass Spectrometry: Beyond Fungal Species Identification.
作者信息
Rizzato Cosmeri, Lombardi Lisa, Zoppo Marina, Lupetti Antonella, Tavanti Arianna
机构信息
Department of Biology, University of Pisa, Via San Zeno 37, 56127 Pisa, Italy.
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via San Zeno 37, 56127 Pisa, Italy.
出版信息
J Fungi (Basel). 2015 Oct 16;1(3):367-383. doi: 10.3390/jof1030367.
Abstract
Matrix assisted laser desorption ionization time of flight (MALDI-TOF) is a powerful analytical tool that has revolutionized microbial identification. Routinely used for bacterial identification, MALDI-TOF has recently been applied to both yeast and filamentous fungi, confirming its pivotal role in the rapid and reliable diagnosis of infections. Subspecies-level identification holds an important role in epidemiological investigations aimed at tracing virulent or drug resistant clones. This review focuses on present and future applications of this versatile tool in the clinical mycology laboratory.
摘要
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）是一种强大的分析工具，它彻底改变了微生物鉴定的方式。MALDI-TOF通常用于细菌鉴定，最近已应用于酵母和丝状真菌，证实了其在感染快速可靠诊断中的关键作用。亚种水平的鉴定在旨在追踪有毒或耐药克隆的流行病学调查中起着重要作用。本综述重点关注这种多功能工具在临床真菌学实验室中的当前和未来应用。
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