Huang Yanfei, Zhang Mingxin, Zhu Min, Wang Mei, Sun Yufeng, Gu Haitong, Cao Jingjing, Li Xue, Zhang Shaoya, Wang Jinglin, Lu Xinxin
Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, 1 Dongjiaominxiang Road, Dongcheng District, Beijing, 100730, China.
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, Beijing, 100071, China.
World J Microbiol Biotechnol. 2017 Jul;33(7):142. doi: 10.1007/s11274-017-2297-3. Epub 2017 Jun 12.
Infections caused by filamentous fungi have become a health concern, and require rapid and accurate identification in order for effective treatment of the pathogens. To compare the performance of two MALDI-TOF MS systems (Bruker Microflex LT and Xiamen Microtyper) in the identification of filamentous fungal species. A total of 374 clinical filamentous fungal isolates sequentially collected in the Clinical Laboratory at the Beijing Tongren Hospital between January 2014 and December 2015 were identified by traditional phenotypic methods, Bruker Microflex LT and Xiamen Microtyper MALDI-TOF MS, respectively. The discrepancy between these methods was resolved by sequencing for definitive identification. Bruker Microflex LT and Xiamen Microtyper had similar correct species ID (98.9 vs. 99.2%), genus ID (99.7 vs. 100%), mis-ID (0.3 vs. 0%) and no ID (0 vs. 0). The rate of correct species identification by both MALDI-TOF MS (98.9 and 99.2%, respectively) was much higher compared with phenotypic approach (91.9%). Both MALDI-TOF MS systems provide accurate identification of clinical filamentous fungi compared with conventional phenotypic method, and have the potential to replace identification for routine identification of these fungi in clinical mycology laboratories. Both systems have similar performance in the identification of clinical filamentous fungi.
丝状真菌引起的感染已成为一个健康问题，为有效治疗病原体，需要快速准确的鉴定。为比较两种基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统（布鲁克Microflex LT和厦门微生物质谱仪）在丝状真菌物种鉴定中的性能。2014年1月至2015年12月期间在北京同仁医院临床实验室连续收集的374株临床丝状真菌分离株，分别采用传统表型方法、布鲁克Microflex LT和厦门微生物质谱仪进行鉴定。这些方法之间的差异通过测序进行最终鉴定来解决。布鲁克Microflex LT和厦门微生物质谱仪的正确物种鉴定率相似（98.9%对99.2%），属鉴定率相似（99.7%对100%），错误鉴定率相似（0.3%对0%），未鉴定率相似（0对0）。两种MALDI-TOF MS的正确物种鉴定率（分别为98.9%和99.2%）均远高于表型方法（91.9%）。与传统表型方法相比，两种MALDI-TOF MS系统均能准确鉴定临床丝状真菌，并且有潜力在临床真菌学实验室替代常规鉴定这些真菌的方法。两种系统在临床丝状真菌鉴定方面具有相似的性能。
