Efimenko N V, Frolkov V K, Kozlova V V, Kaisinova A S, Chalaya E N
Pyatigorsk State Scientific and Research Institute of Balneology.
Russian Research Center of Medical Rehabilitation and Balneology.
Vopr Kurortol Fizioter Lech Fiz Kult. 2017 Dec 5;94(5):25-29. doi: 10.17116/kurort201794525-29.
The objective of the present research was to study the influence of tap water enriched with silver nanoparticles (NP) as well as that of «Krasnoarmeysky» and «Essentuki №17» mineral waters after their single administration through the oral gavage to the rats on the metabolism of carbohydrates and lipids, the biochemical markers of the liver condition, and the endocrine profile in the healthy animals.
The laboratory animals (130 male Wistar rats) were allocated to thirteen groups comprised of 10 rats each as follows: 1st group (n=10) intact animals, 2nd group (5 minutes after the administration of silver NP (n=10), 3rd group (15 minutes after the of silver NP), 4th group (60 minutes after the administration of silver NP), 5th group (n=10) (5 minutes after the introduction of the «Krasnoarmeysky» mineral water), 6th group (n=10) (15 min after the introduction of the «Krasnoarmeysky» mineral water), 7th group (n=10), (60 minutes after the introduction of the «Krasnoarmeysky» mineral water) 8th group (n=10) (5 minutes after the introduction of the «Essentuki № 17» mineral water), 9th group (n=10) (15 min after the introduction of the «Essentuki № 7» mineral water) , 10th group (n=10) (60 minutes after the introduction of the «Essentuki №17» mineral water), 11th group (n=10) (5 minutes after administration of tap water (control),12th group (n=10) (15 minutes after administration of tap water (control), and 13th (n=10) group 60 minutes after administration of tap water (control).
The study has demonstrated that the tap water enriched with silver nanoparticles similar to the mineral waters caused stress reactions that are inferior to those induced by «Essentuki №17» mineral water in terms of the magnitude; however, the effect provoked by the tap water was of longer duration. Moreover, the tap water enriched with silver nanoparticles stimulates prooxidant reactions, and inhibit the activity of antioxidant protection. Silver nanoparticles appear to produce some destructive effect on the hepatocytes.
The silver nanoparticles present in the tap water have a significant biological potential of their own. Moreover, their one-time action is apt to alter the biological potential of the water into which they are administered. The single intake of the tap water enriched with silver nanoparticles by the healthy laboratory animals produces the response that resembles that of the drinking mineral water.
本研究的目的是研究经口灌胃给予大鼠单次含银纳米颗粒的自来水以及“Krasnoarmeysky”和“Essentuki №17”矿泉水后，对健康动物碳水化合物和脂质代谢、肝脏状况生化标志物以及内分泌谱的影响。
将实验动物（130只雄性Wistar大鼠）分为13组，每组10只大鼠，分组如下：第1组（n = 10）为正常动物；第2组（给予银纳米颗粒后5分钟，n = 10）；第3组（给予银纳米颗粒后15分钟）；第4组（给予银纳米颗粒后60分钟）；第5组（n = 10）（给予“Krasnoarmeysky”矿泉水后5分钟）；第6组（n = 10）（给予“Krasnoarmeysky”矿泉水后15分钟）；第7组（n = 10）（给予“Krasnoarmeysky”矿泉水后60分钟）；第8组（n = 10）（给予“Essentuki №17”矿泉水后5分钟）；第9组（n = 10）（给予“Essentuki №7”矿泉水后15分钟）；第10组（n = 10）（给予“Essentuki №17”矿泉水后60分钟）；第11组（n = 10）（给予自来水后5分钟（对照））；第12组（n =
10）（给予自来水后15分钟（对照））；第13组（n = 10）（给予自来水后60分钟（对照））。
研究表明，含银纳米颗粒的自来水与矿泉水一样会引起应激反应，但其强度低于“Essentuki №17”矿泉水引起的应激反应；然而，自来水引起的效应持续时间更长。此外，含银纳米颗粒的自来水会刺激促氧化反应，并抑制抗氧化保护活性。银纳米颗粒似乎对肝细胞产生了一些破坏作用。
自来水中存在的银纳米颗粒具有显著的自身生物学潜能。此外，它们的一次性作用易于改变其所加入水中的生物学潜能。健康实验动物单次摄入含银纳米颗粒的自来水会产生类似于饮用矿泉水的反应。
