Baris O, Demir T, Gulluce M
Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
Niger J Clin Pract. 2017 Dec;20(12):1571-1575. doi: 10.4103/1119-3077.187316.
Although it is known that bacterial mechanisms are involved in dental calculus formation, which is a predisposing factor in periodontal diseases, there have been few studies of such associations, and therefore, information available is limited. The purpose of this study was to isolate and identify aerobic bacteria responsible for direct calcification from supragingival calculus samples.
The study was conducted using supragingival calculus samples from patients with periodontal disease, which was required as part of conventional treatment. Isolations were performed by sampling the supragingival calculus with buffer and inoculating the samples on media on which crystallization could be observed. The 16S recombinant DNA of the obtained pure cultures was then amplified and sequenced.
A few bacterial species that have not previously been associated with mineralization or identified on bacterial plaque or calculus were detected. The bacteria that caused mineralization an aerobic environment are identified as Neisseria flava, Aggregatibacter segnis, Streptococcus tigurinus, and Morococcus cerebrosus.
These findings proved that bacteria potentially play a role in the etiopathology of supragingival calculus. The association between the effects of the identified bacteria on periodontal diseases and calculus formation requires further studies.
虽然已知细菌机制参与牙菌斑的形成，而牙菌斑是牙周疾病的一个诱发因素，但关于此类关联的研究较少，因此，现有信息有限。本研究的目的是从龈上牙石样本中分离并鉴定负责直接钙化的需氧菌。
本研究使用了牙周疾病患者的龈上牙石样本，这是常规治疗所需的。通过用缓冲液对龈上牙石进行采样，并将样本接种到可观察到结晶的培养基上进行分离。然后对获得的纯培养物的16S重组DNA进行扩增和测序。
检测到一些以前未与矿化相关或在牙菌斑或牙石中鉴定出的细菌种类。在需氧环境中导致矿化的细菌被鉴定为微黄奈瑟菌、惰性聚集杆菌、蒂氏链球菌和脑心莫拉菌。
这些发现证明细菌可能在龈上牙石的病因学中起作用。已鉴定细菌对牙周疾病的影响与牙石形成之间的关联需要进一步研究。
