Sharma Deepika, Sharma Shikha, Pal Vijay, Lal Rup, Patil Prabhu, Grover Vishakha, Korpole Suresh
1MTCC and Gene Bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
2The Energy and Resources Institute, New Delhi, India.
Indian J Microbiol. 2020 Mar;60(1):78-86. doi: 10.1007/s12088-019-00828-8. Epub 2019 Oct 8.
Human oral cavity is a complex habitat comprising about 700 microbial species and represents the most complex microbiota after gastrointestinal tract. In fact, oral microbiota directly influences health, metabolism and immune responses of the host. Metagenomic studies based on 16S rDNA profiling has reported the inhabitant bacteria mainly belonging to phyla , , , , and . Therefore, it is essential to isolate these strains and characterize in detail to understand their interaction. We have isolated strains from subgingival plaque from healthy to diseased individuals and the molecular characterization based on 16S rRNA gene sequence analysis showed predominance of , specifically members of the genus . Species of and were also found in significant number, which are considered as secondary colonizers. However, the population of was decreased in diseased conditions with the increase in opportunistic pathogenic strains pertaining to genera like , , , and . Further, we have also made an attempt to gain genomic insights on adaptation features and interactions of an isolate, sp. strain DISK7 by performing whole genome sequencing and analysis, subsequently biochemical characterization to explore its functional and metabolic properties for the development as probiotic agent.
人类口腔是一个复杂的栖息地，包含约700种微生物，是继胃肠道之后最复杂的微生物群。事实上，口腔微生物群直接影响宿主的健康、新陈代谢和免疫反应。基于16S rDNA分析的宏基因组学研究报告称，口腔中的细菌主要属于门、、、、和。因此，分离这些菌株并进行详细表征以了解它们之间的相互作用至关重要。我们从健康个体到患病个体的龈下菌斑中分离出菌株，基于16S rRNA基因序列分析的分子表征显示，主要以菌属成员为主。和菌属的物种数量也较多，它们被认为是次级定植菌。然而，在患病情况下，随着与、、、和等菌属相关的机会致病菌数量增加，菌的数量减少。此外，我们还通过全基因组测序和分析，对分离株sp.菌株DISK7的适应特征和相互作用进行了基因组学研究，随后进行生化表征，以探索其功能和代谢特性，用于开发益生菌制剂。
