Department of Chemistry, Capital Normal University, Beijing 100048, P. R. China.
Lab Chip. 2018 Feb 13;18(4):610-619. doi: 10.1039/c7lc01234a.
Point-of-care (POC), sample-to-answer and electricity-free nucleic acid diagnostic tools are vital for health care and disease control in resource-limited settings where centralized medical facilities or even electric power may remain unreliable. Inspired by one of the oldest recognizable toys, the spinning top, here we report a fully hand-powered centrifugal microfluidic platform for the diagnostics of pathogenic bacteria. Assay procedures such as zeolite-based purification of nucleic acids, loop-mediated isothermal amplification (LAMP) and visual detection of fluorescence signals are integrated into a single microfluidic disc. A simple pull-out operation of the top rack of the customized centrifuge initiates high-speed rotation of the disc, resulting in efficient actuation and mixing of preloaded sample/reagent fluids. This microfluidic platform enables the simultaneous detection of six kinds of pathogenic bacteria within a small disc in an electricity-free manner, showing great promise in sample-to-answer nucleic acid detection in remote settings.
即时检测（POC）、样本到答案和无电源核酸诊断工具对于资源有限环境中的医疗保健和疾病控制至关重要，在这些环境中，集中式医疗设施甚至电力可能仍然不可靠。受最古老的可识别玩具之一——陀螺的启发，我们在此报告了一种完全手动驱动的离心微流控平台，用于诊断病原菌。基于沸石的核酸纯化、环介导等温扩增（LAMP）和荧光信号的目视检测等检测程序被整合到单个微流控盘中。通过定制离心机的顶架的简单拉出操作，启动圆盘的高速旋转，从而有效地启动和混合预加载的样品/试剂流体。这种微流控平台能够在无电源的情况下在一个小盘中同时检测六种病原菌，在远程环境中的样本到答案核酸检测方面具有广阔的应用前景。
