Cheng Bill, Chao Wei-Cheng, Chen Yi-Han, Lin Yao-Tsung
Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan.
Doctoral Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan.
Front Bioeng Biotechnol. 2025 Aug 1;13:1611313. doi: 10.3389/fbioe.2025.1611313. eCollection 2025.
Using microfluidic flow for biological detection is a non-invasive method that can replace traditional invasive testing methods to achieve fast and accurate results. The design of the detection device and lab-on-a-disk (LoaD) can impact performance in accurately identifying biological features. Therefore, we created a novel device to extract cancer cells from a heterogeneous cell population by centrifugal-force-driven microfluidic flow and magnetic labeling. Two-stage centrifugal force and a specially designed LoaD were used to drive microfluidic flow and control its movement to designated areas. The purpose was to allow the CD44 antibody-magnetic bead complex (CD44 beads), which specifically binds to the abundantly present CD44 receptors on identifiable cancer cells, to flow into the reservoir well, while the biological mixture containing the cancer cells is retained in the capture well. Fluorescence imaging as well as flow cytometric analysis revealed the successful retention of the microbead-bound cancer cells in the magnetic area, while the remaining biological mixture was retained in the reservoir area. The entire separation process took less than 2 h.
利用微流体流动进行生物检测是一种非侵入性方法，它可以取代传统的侵入性检测方法，以实现快速、准确的结果。检测装置和芯片实验室（LoaD）的设计会影响准确识别生物特征的性能。因此，我们创建了一种新型装置，通过离心力驱动的微流体流动和磁性标记，从异质细胞群体中提取癌细胞。利用两级离心力和专门设计的LoaD来驱动微流体流动，并将其运动控制到指定区域。目的是使与可识别癌细胞上大量存在的CD44受体特异性结合的CD44抗体-磁珠复合物（CD44磁珠）流入储液孔，而含有癌细胞的生物混合物则保留在捕获孔中。荧光成像以及流式细胞术分析表明，与微珠结合的癌细胞成功保留在磁性区域，而其余生物混合物则保留在储液区域。整个分离过程耗时不到2小时。
