Department of Chemistry, Tsinghua University, Beijing 100084, China.
The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Sensors (Basel). 2018 Jan 29;18(2):385. doi: 10.3390/s18020385.
The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells.
蛋白质的检测在疾病的早期诊断、预后跟踪和治疗评估中作为生物标志物具有重要意义。因此，我们开发了一种基于肽和氧化石墨烯（GO）的简单、灵敏、通用的蛋白质传感平台。该设计由荧光团（TAMRA，TAM）、含有八个精氨酸的肽和可识别靶蛋白的肽配体以及用作猝灭剂的 GO 组成。为了证明传感器在目标检测中的可行用途，Bcl-xL 被评估为模型靶标。该传感器具有灵敏度高的特点，并应用于缓冲液、2%血清和活细胞中目标蛋白的检测。
