Noble N A, Rothstein G
Blood. 1986 May;67(5):1210-4.
The genetic locus designated Dpg has two alleles in outbred Long-Evans rats. Genotype at this locus affects quantities of red cell 2,3- diphosphoglycerate (DPG) and adenosine triphosphate, as well as activities of two important glycolytic enzymes: phosphofructokinase and pyruvate kinase. Intravascular red cell survival is shortened in low-DPG animals. In order to get closer to the specific action of this locus, we addressed the question of whether the Dpg gene acts through intracorpuscular or extracorpuscular factors. Bone marrow transplantation after total body irradiation and 51Cr red cell survival after cross transfusion were the methods used. Because the animals that were used differed in hemoglobin phenotype, donor and recipient cells could be quantified in cross-transplanted animals. Phenotypic markers of Dpg genotype were measured in animals 40 to 50 days after transplantation. Values for these markers correlated highly with the percentage of donor and recipient cells present. In vivo survival of low-DPG red cells was significantly shorter than that of high-DPG cells (P less than .05), regardless of the genotype of the recipient. From the present studies, we conclude that the action of the Dpg gene is exerted by an intracorpuscular factor.
在远交群长-伊文斯大鼠中，名为Dpg的基因座有两个等位基因。该基因座的基因型会影响红细胞2,3-二磷酸甘油酸（DPG）和三磷酸腺苷的含量，以及两种重要糖酵解酶的活性：磷酸果糖激酶和丙酮酸激酶。低DPG动物的血管内红细胞存活时间缩短。为了更深入了解该基因座的具体作用，我们探讨了Dpg基因是通过细胞内还是细胞外因素发挥作用的问题。采用的方法是全身照射后的骨髓移植以及交叉输血后的51Cr红细胞存活实验。由于所使用的动物血红蛋白表型不同，因此可以对交叉移植动物体内的供体和受体细胞进行定量分析。在移植后40至50天的动物中测量Dpg基因型的表型标记物。这些标记物的值与供体和受体细胞的百分比高度相关。无论受体的基因型如何，低DPG红细胞在体内的存活时间均显著短于高DPG红细胞（P小于0.05）。从目前的研究中，我们得出结论，Dpg基因的作用是由细胞内因素介导的。
