Laboratory of Hygienic Chemistry and Molecular Toxicology, Gifu Pharmaceutical University.
Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science.
Biol Pharm Bull. 2018;41(2):266-271. doi: 10.1248/bpb.b17-00830.
Corn oil, sesame oil, and 10% ethanol in corn oil are commonly used as dosing vehicles in toxicology studies. Since these vegetable oils contain bioactive compounds, it is important for toxicology studies to characterize the toxicities of the dosing vehicles themselves. It has been recently proposed that the width of the genital tubercle (GT), the dorsal-ventral length (D-V length) of the GT, and urethral tube closure in mouse fetuses can be used as novel markers for monitoring sexual development in mice. However, how these parameters are influenced by the dosing vehicles themselves remains unclear. Therefore, we evaluated the effects of corn oil, sesame oil, and 10% ethanol in corn oil on GT width, D-V length, and GT morphology in ICR mice. Our results showed that all three vehicles influenced GT width and D-V length, but not GT morphology, suggesting that the effects of dosing vehicles themselves might need to be considered when GT width or D-V length is used as a parameter to evaluate the effects of chemicals on GT development.
玉米油、芝麻油和 10%玉米油中的乙醇通常用作毒理学研究中的给药载体。由于这些植物油含有生物活性化合物，因此对于毒理学研究来说，描述给药载体本身的毒性非常重要。最近有人提出，小鼠胎儿的生殖器嵴（GT）宽度、GT 的背-腹长度（D-V 长度）和尿道管闭合可以作为监测小鼠性发育的新标志物。然而，这些参数如何受到给药载体本身的影响尚不清楚。因此，我们评估了玉米油、芝麻油和 10%玉米油中的乙醇对 ICR 小鼠 GT 宽度、D-V 长度和 GT 形态的影响。我们的结果表明，这三种载体都影响 GT 宽度和 D-V 长度，但不影响 GT 形态，这表明在将 GT 宽度或 D-V 长度用作评估化学物质对 GT 发育影响的参数时，可能需要考虑给药载体本身的影响。
