Fujimoto Kota, Hashimoto Daiki, Kim Sang Woon, Lee Yong Seung, Suzuki Takuya, Nakata Masanori, Kumegawa Shinji, Asamura Shinichi, Yamada Gen
Department of Developmental Genetics, Institute of Advanced Medicine Wakayama Medical University Wakayama Japan.
Department of Plastic and Reconstructive Surgery Wakayama Medical University Wakayama Japan.
Reprod Med Biol. 2024 Apr 1;23(1):e12570. doi: 10.1002/rmb2.12570. eCollection 2024 Jan-Dec.
The pathophysiology of penis extends to erectile dysfunction (ED) to conditions including sexually transmitted diseases (STDs) and cancer. To date, there has been little research evaluating vascular drainage from the penis. We aimed to evaluate penile blood flow in vivo and analyze its possible relationship with the lymphatic maker.
We established an in vivo system designed to assess the dynamic blood outflow from the corpus cavernosum (CC) by dye injection. To analyze lymphatic characteristics in the CC, the expression of Lyve-1, the key lymphatic endothelium marker, was examined by the in vitro system and lipopolysaccharide (LPS) injection to mimic the inflammatory conditions.
A novel cavernography methods enable high-resolution morphological and functional blood drainage analysis. The expression of Lyve-1 was detected along the sinusoids. Furthermore, its prominent expression was also observed after penile LPS injection and in the erectile condition.
The current in vivo system will potentially contribute to the assessment of penile pathology from a novel viewpoint. In addition, current analyses revealed inducible Lyve-1 expression for LPS injection and the erection state, which requires further analyses on penile lymphatic system.
阴茎的病理生理学延伸至勃起功能障碍（ED）以及包括性传播疾病（STD）和癌症在内的病症。迄今为止，很少有研究评估阴茎的血管引流情况。我们旨在评估阴茎的体内血流，并分析其与淋巴管标志物的可能关系。
我们建立了一个体内系统，通过染料注射来评估海绵体（CC）的动态血液流出。为了分析CC中的淋巴管特征，通过体外系统和注射脂多糖（LPS）以模拟炎症状态来检测关键淋巴管内皮标志物Lyve-1的表达。
一种新型的海绵体造影方法能够进行高分辨率的形态学和功能性血液引流分析。沿窦状隙检测到Lyve-1的表达。此外，在阴茎注射LPS后以及勃起状态下也观察到其显著表达。
当前的体内系统可能会从一个新的角度为阴茎病理学的评估做出贡献。此外，目前的分析揭示了LPS注射和勃起状态下可诱导的Lyve-1表达，这需要对阴茎淋巴系统进行进一步分析。
