Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2023 Dec 5;24(24):17164. doi: 10.3390/ijms242417164.
Verticillium wilt is a soil-borne vascular disease caused by the fungal pathogen . It causes great harm to upland cotton () yield and quality. A previous study has shown that Hen egg white lysozyme (HEWL) exerts strong inhibitory activity against in vitro. In the current study, we introduced the gene into cotton through the -mediated transformation, and the exogenous HEWL protein was successfully expressed in cotton. Our study revealed that HEWL was able to significantly inhibit the proliferation of in cotton. Consequently, the overexpression of HEWL effectively improved the resistance to Verticillium wilt in transgenic cotton. In addition, ROS accumulation and NO content increased rapidly after the inoculation of plant leaves overexpressing HEWL. In addition, the expression of the PR genes was significantly up-regulated. Taken together, our results suggest that HEWL significantly improves resistance to Verticillium wilt by inhibiting the growth of pathogenic fungus, triggering ROS burst, and activating PR genes expression in cotton.
黄萎病是一种由真菌病原体引起的土壤传播的血管疾病。它对旱地棉花的产量和质量造成了极大的危害。先前的研究表明，蛋清溶菌酶（HEWL）对离体具有很强的抑制活性。在本研究中，我们通过农杆菌介导的转化将基因导入棉花，外源 HEWL 蛋白在棉花中成功表达。我们的研究表明，HEWL 能够显著抑制在棉花中的增殖。因此，HEWL 的过表达有效提高了转基因棉花对黄萎病的抗性。此外，在过表达 HEWL 的植物叶片接种后，ROS 积累和 NO 含量迅速增加。此外，PR 基因的表达明显上调。综上所述，我们的研究结果表明，HEWL 通过抑制病原菌的生长、触发 ROS 爆发和激活棉花中 PR 基因的表达，显著提高了对黄萎病的抗性。
