Ji Yunrui, Mou Minghui, Zhang Huimin, Wang Ruling, Wu Songguo, Jing Yifen, Zhang Haiyan, Li Lanxin, Li Zhifang, Chen Ligang
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Plant Divers. 2022 Apr 14;45(3):337-346. doi: 10.1016/j.pld.2022.04.001. eCollection 2023 May.
wilt, caused by , seriously restricts the yield and quality improvement of cotton. Previous studies have revealed the involvement of WRKY members in plant defense against , but the underlying mechanisms involved need to be further elucidated. Here, we demonstrated that WRKY DNA-binding protein 33 () functions as a negative regulator in plant defense against . expression is induced rapidly by and methyl jasmonate, and overexpression of reduces plant tolerance to in . Quantitative RT-PCR analysis revealed that expression of several JA-associated genes was significantly repressed in overexpressing transgenic plants. Yeast one-hybrid analysis revealed that GhWRKY33 may repress the transcription of both and through its binding to their promoters. Protein-protein interaction analysis suggested that GhWRKY33 interacts with JASMONATE ZIM-domain protein 3 (GhJAZ3). Similarly, overexpression of also decreases plant tolerance to . Furthermore, GhJAZ3 acts synergistically with GhWRKY33 to suppress both and expression. Our results imply that may negatively regulate plant tolerance to via the JA-mediated signaling pathway.
枯萎病由[病原体名称未给出]引起，严重限制了棉花产量和品质的提升。先前的研究已揭示WRKY成员参与植物对[病原体名称未给出]的防御，但其中涉及的潜在机制仍需进一步阐明。在此，我们证明WRKY DNA结合蛋白33（GhWRKY33）在植物对[病原体名称未给出]的防御中起负调控作用。GhWRKY33的表达受[病原体名称未给出]和茉莉酸甲酯快速诱导，且GhWRKY33的过表达降低了棉花对[病原体名称未给出]的耐受性。定量逆转录聚合酶链反应分析表明，在过表达GhWRKY33的转基因植物中，几个与茉莉酸相关的基因表达显著受抑制。酵母单杂交分析表明，GhWRKY33可能通过与[基因名称未给出]和[基因名称未给出]的启动子结合来抑制它们的转录。蛋白质-蛋白质相互作用分析表明，GhWRKY33与茉莉酸ZIM结构域蛋白3（GhJAZ3）相互作用。同样，GhJAZ3的过表达也降低了植物对[病原体名称未给出]的耐受性。此外，GhJAZ3与GhWRKY33协同作用以抑制[基因名称未给出]和[基因名称未给出]的表达。我们的结果表明，GhWRKY33可能通过茉莉酸介导的信号通路负调控植物对[病原体名称未给出]的耐受性。
