血清学诊断方法对牛布鲁氏菌病的诊断效能比较及聚合酶链反应诊断评估
Comparative efficacy of serological diagnostic methods and evaluation of polymerase chain reaction for diagnosis of bovine brucellosis.
作者信息
Verma A K, Kumar A, Ahmed I
机构信息
MVSc Scholar, Department of Veterinary Epidemiology and Preventive Medicine, U. P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
Department of Veterinary Epidemiology and Preventive Medicine, U. P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India.
出版信息
Iran J Vet Res. 2017 Fall;18(4):279-281.
The aim of present study was to compare the diagnostic performance of the different antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR and I-ELISA (κ=0.48). A slight degree of agreement was observed between RBPT and I-ELISA and PCR (κ=0.18) and RBPT and PCR (κ=0.11). Indirect enzyme linked immunosorbent assay detected more samples as positive among these tests. In conclusion, I-ELISA can be routinely used for an accurate and efficient diagnosis of infection, because the chances of non-detection of an infected animal in I-ELISA are minimal. However, PCR could be used as a supplement and complement test along with I-ELISA for identification and differentiation of bovine brucellosis.
本研究的目的是比较不同基于抗原的血清学和分子检测方法，如虎红平板凝集试验（RBPT）、间接酶联免疫吸附测定（I-ELISA）和聚合酶链反应（PCR）的诊断性能。在89份样本中，I-ELISA检测出44份阳性，RBPT检测出18份阳性，PCR检测出21份阳性。PCR与I-ELISA之间观察到高度一致性（κ=0.48）。RBPT与I-ELISA及PCR之间观察到轻度一致性（κ=0.18），RBPT与PCR之间观察到轻度一致性（κ=0.11）。在这些检测方法中，间接酶联免疫吸附测定检测出更多阳性样本。总之，I-ELISA可常规用于准确、高效地诊断感染，因为I-ELISA漏检感染动物的可能性极小。然而，PCR可作为I-ELISA的补充和辅助检测方法，用于牛布鲁氏菌病的鉴定和鉴别。
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