Chen Lin, Abudureheman Buhailiqiemu, Anwar Omar, Abdugini Emran, Zhang Jianlin, Tang Rui, Gao Zhihui, Pan Haibo, Ye Xingqian
Center for Experimental Instruction in Food Safety and Nutrition, Xinjiang Institute of Technology, Aksu 843000, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Foods. 2025 May 21;14(10):1823. doi: 10.3390/foods14101823.
L., is a species of traditional Chinese medicine herbs, widely distributed northwestern China and used as antidiabetic, antibacterial etc., but the active compounds and their abundance have not been systematically investigated. This research focused on the following: (i) optimizing polyphenol extraction/purification from ; (ii) UPLC-QE-based profiling of polyphenolic composition; (iii) FT-IR-assisted structural elucidation; and (iv) functional assessment of antioxidant and antibacterial properties. The results showed that the highest extraction yield of crude polyphenols of (CPA) reached 5.02 ± 0.04% at an ethanol concentration of 70% of 70 °C with a solid-to-liquid ratio of 1:20 (g/mL). The D101 macroporous resin is the best one for polyphenolpurification of (PPA), with a purification efficiency of 60.48 ± 1.87%. UPLC-QE analysis identified 36 polyphenolic compounds in PPA, in whic the content of protocatechuic acid is the highest at 1338.05 ± 1.83 ng/mg. The absorption peaks at 1691 cm (carbonyl, C=O), 1605 cmand 1518 cm (aromatic C=C), as well as 1275 cm and 1369 cm (C-O stretching), indicated the presence of phenolic acids, flavonoids and tannins in PPA by FT-IR. PPA exhibited significant antioxidant activity, which reached 81.73 ± 1.43% for DPPH, 87.11 ± 1.57% for hydroxyl and 85.74 ± 1.52% for ABTS+. It also demonstrated strong antibacterial activity against nine common pathogenic bacteria, but not to Escherichia coli. polyphenols demonstrate potent bioactive properties, suggesting potential applications in functional foods and natural preservatives.
L.是一种传统中药，在中国西北部广泛分布，可用作抗糖尿病、抗菌等药物，但其中的活性化合物及其含量尚未得到系统研究。本研究聚焦于以下几点：（i）优化从L.中提取/纯化多酚的方法；（ii）基于超高效液相色谱-四极杆飞行时间质谱（UPLC-QE）对多酚成分进行分析；（iii）利用傅里叶变换红外光谱（FT-IR）辅助进行结构解析；（iv）对抗氧化和抗菌性能进行功能评估。结果表明，在乙醇浓度为70%、温度为70℃、固液比为1:20（g/mL）的条件下，L.粗多酚（CPA）的最高提取率达到5.02±0.04%。D101大孔树脂是纯化L.多酚（PPA）的最佳树脂，纯化效率为60.48±1.87%。UPLC-QE分析鉴定出PPA中有36种多酚化合物，其中原儿茶酸含量最高，为1338.05±1.83 ng/mg。在1691 cm（羰基，C=O）、1605 cm和1518 cm（芳香族C=C）以及1275 cm和1369 cm（C-O伸缩振动）处的吸收峰，通过FT-IR表明PPA中存在酚酸、黄酮类化合物和单宁。PPA表现出显著的抗氧化活性，对二苯基苦味酰基自由基（DPPH）的清除率达到81.73±1.43%，对羟基自由基的清除率为87.11±1.57%，对ABTS+自由基的清除率为85.74±1.52%。它还对9种常见病原菌表现出较强的抗菌活性，但对大肠杆菌无抗菌活性。L.多酚具有强大的生物活性，表明其在功能性食品和天然防腐剂方面具有潜在应用价值。
