Matsuda Akiko, Patel Tushar
Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA.
Department of Cancer Biology, Mayo Clinic, Jacksonville, FL, USA.
Methods Mol Biol. 2018;1740:187-197. doi: 10.1007/978-1-4939-7652-2_15.
As endogenous biological nanoparticles capable of uptake by cells, extracellular vesicles (EVs) have the capacity to deliver their RNA cargo to recipient cells. The use of EVs as a drug delivery system remains in its infancy, and there are several barriers to the use of EV for this purpose. Amongst these is the need to ensure that adequate amounts of EV are available. The use of milk-derived EV provides a scalable approach and loading of these EVs with RNA is possible with the use of chemical transfection reagents. This method describes the use of milk-derived EV for delivery of small interfering RNA. These EVs were shown to be taken up by hepatocellular carcinoma cells in vitro, with a reduction in the expression of target gene.
作为能够被细胞摄取的内源性生物纳米颗粒，细胞外囊泡（EVs）有能力将其RNA货物递送至受体细胞。将EVs用作药物递送系统仍处于起步阶段，为此目的使用EVs存在若干障碍。其中之一是需要确保有足够数量的EVs可用。使用源自牛奶的EVs提供了一种可扩展的方法，并且通过使用化学转染试剂可以将这些EVs装载RNA。本方法描述了使用源自牛奶的EVs递送小干扰RNA。这些EVs在体外被证明可被肝癌细胞摄取，同时靶基因的表达降低。
