通过细胞外囊泡在体外和体内递送功能性小 RNA。
Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo.
机构信息
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Boston University, Boston, MA, USA.
出版信息
Methods Mol Biol. 2020;2115:107-117. doi: 10.1007/978-1-0716-0290-4_6.
Extracellular vesicles (EVs) are naturally generated nanovesicles which potentially mediate the intercellular communication and interorgan crosstalk. EVs have recently gained significant interest as a promising material for delivery of therapeutics. Small RNAs, including small interfering RNA (siRNA) and microRNA (miRNA), provide a great therapeutic strategy for treating human diseases. However, it remains a challenge to deliver unconjugated small RNAs to the target tissue or cells. The delivery of small RNAs in an EV-encapsulating manner has a number of advantages, such as enhancing the concentration of small RNAs, improving the uptake of small RNAs by the recipient cells, and potentially achieving a cell-specific delivery. In this chapter, a protocol is provided for EV preparation and loading with small RNAs. Additionally, a detailed experimental protocol for tracking and validating small RNA delivery into the lungs is described. Overall, the described protocols are valuable for delivering functional small RNAs both in vitro and in vivo.
细胞外囊泡（EVs）是天然产生的纳米囊泡，可能介导细胞间通讯和器官间串扰。EVs 最近作为治疗药物的有前途的材料引起了广泛关注。小 RNA，包括小干扰 RNA（siRNA）和 microRNA（miRNA），为治疗人类疾病提供了一种很好的治疗策略。然而，将未缀合的小 RNA 递送到靶组织或细胞仍然是一个挑战。以 EV 包裹的方式递送小 RNA 具有许多优点，例如提高小 RNA 的浓度、增加受体细胞对小 RNA 的摄取，并有可能实现细胞特异性递送。在本章中，提供了一种 EV 制备和加载小 RNA 的方案。此外，还描述了一种详细的实验方案，用于跟踪和验证小 RNA 递送到肺部。总的来说，所描述的方案对于在体外和体内递送功能性小 RNA 是有价值的。
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