Argenti L E, Parmeggiani B S, Leipnitz G, Weber A, Pereira G R, Bustamante-Filho I C
Biotechnology Laboratory, Post-Graduate Program in Biotechnology, Universidade do Vale do Taquari - UNIVATES, Lajeado, RS, Brazil.
Biochemistry Department, Post-Graduate Program in Biological Sciences: Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Andrologia. 2018 Feb 1. doi: 10.1111/and.12951.
Although boar semen productivity is affected by seasonality, its effects are not equal among different regions which raise concerns regarding the profitability of boar stud farms. Therefore, the goals of this study were (i) to evaluate the seasonal effect on semen production in a commercial boar stud farm located in a subtropical climate region and (ii) to verify whether the activities of superoxide dismutase and glutathione peroxidase in spermatozoa and seminal plasma were associated with seminal traits of fresh and cooled semen. Nine boars were collected twice per season, and routine seminal parameter analyses were performed together with superoxide dismutase and glutathione peroxidase activities in seminal plasma and spermatozoa. Despite a reduction in sperm concentration in spring and summer, most seminal parameters were constant year-round. Temperature-humidity index was higher in the summer compared to spring, autumn and winter (p < .05). Superoxide dismutase activity in spermatozoa was increased in summer compared to autumn and winter (p < .05). The activities of both enzymes in seminal plasma and spermatozoa glutathione peroxidase remained unaltered throughout the seasons. In conclusion, seasonality showed little influence in overall boar seminal parameters despite microclimatic differences among seasons, and spermatozoa collected during summer increased superoxide dismutase activity.
尽管公猪精液产量受季节性影响，但其影响在不同地区并不相同，这引发了对公猪精液站盈利能力的担忧。因此，本研究的目的是：（i）评估位于亚热带气候地区的商业化公猪精液站精液生产的季节性影响；（ii）验证精子和精浆中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性是否与新鲜精液和冷藏精液的精液特性相关。每个季节采集9头公猪的精液两次，并对精液进行常规参数分析，同时检测精浆和精子中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。尽管春季和夏季精子浓度有所下降，但大多数精液参数全年保持稳定。与春季、秋季和冬季相比，夏季的温度-湿度指数更高（p < 0.05）。与秋季和冬季相比，夏季精子中的超氧化物歧化酶活性增加（p < 0.05）。精浆和精子中谷胱甘肽过氧化物酶的活性在四季中均保持不变。总之，尽管季节间存在小气候差异，但季节性对公猪精液总体参数影响不大，夏季采集的精子超氧化物歧化酶活性增加。
