Lahouar Amani, Jedidi Ines, Sanchis Vicente, Saïd Salem
a Laboratory of Biochemistry, Unity of Mycotoxicology , University of Sousse , Sousse , Tunisia.
b Food Technology Department , University of Lleida , Lleida , Spain.
Food Addit Contam Part B Surveill. 2018 Jun;11(2):103-110. doi: 10.1080/19393210.2018.1433239. Epub 2018 Mar 2.
A total of 64 samples of sorghum (37 Tunisian sorghum samples and 27 Egyptian sorghum samples) were collected during 2011-2012 from markets in Tunisia. Samples were analysed for contamination with aflatoxin B1, ochratoxin A and zearalenone by High-Performance Liquid Chromatography Coupled with Fluorescence Detection (HPLC-FLD). Aflatoxin B1 was found in 38 samples in the range 0.03-31.7 µg kg. Ochratoxin A was detected in 24 samples with concentrations ranging from 1.04 to 27.8 µg kg. Zearalenone was detected in 21 samples and the concentration varied between 3.7 and 64.5 µg kg. ANOVA analysis of the influence of the country of origin on the incidence and concentration of mycotoxins in the samples studied showed no significant difference (P > 0.05) between the two batches of samples for each of the three mycotoxins studied. The studied mycotoxins contaminate sorghum and may also co-exist because of the diversity of the mycobiota in this cereal.
2011年至2012年期间,从突尼斯的市场上总共采集了64份高粱样本(37份突尼斯高粱样本和27份埃及高粱样本)。采用高效液相色谱-荧光检测法(HPLC-FLD)对样本中的黄曲霉毒素B1、赭曲霉毒素A和玉米赤霉烯酮污染情况进行分析。在38份样本中检测到黄曲霉毒素B1,含量范围为0.03至31.7微克/千克。在24份样本中检测到赭曲霉毒素A,浓度范围为1.04至27.8微克/千克。在21份样本中检测到玉米赤霉烯酮,浓度在3.7至64.5微克/千克之间。对原产国对所研究样本中霉菌毒素的发生率和浓度的影响进行方差分析表明,所研究的三种霉菌毒素中的每一种,两批样本之间均无显著差异(P>0.05)。所研究的霉菌毒素会污染高粱,并且由于这种谷物中真菌群落的多样性,它们也可能共存。