Subhash Y, Lee Sang-Seob
Department of Life Science, College of Natural Science, Kyonggi University, 154-42 Gwanggyosan-ro Yeongtong-gu Suwon-Si, Gyeonggi-Do, 16227, Republic of Korea.
Present address: Royal Netherlands Institute for Sea Research (NIOZ), Texel, The Netherlands.
Int J Syst Evol Microbiol. 2018 Feb;68(2):675-680. doi: 10.1099/ijsem.0.002565.
Two dark pink pigmented bacterial strains (M3 and M11) were isolated from crude oil contaminated desert sand from Kuwait. Both strains were Gram-stain-negative and small-rod to oval-shaped bacteria. Strains M3 and M11 grew at 13-42 °C (optimum, 30-35 °C) and pH 6.5-9.0 (optimum, 7.0-7.5). No additional NaCl was required for the growth of both strains. The genomic DNA G+C content of strains M3 and M11 were 69.5 and 69.0 mol%, respectively. Both strains were closely related and the mean DNA-DNA hybridization value was 92±1 %. 16S rRNA gene sequence comparisons of both strains indicated that they belong to the genus Roseomonas. Strains M3 and M11 had a sequence similarity of 97.3 and 97.4 % with Roseomonas oryzae JC288, respectively. Both strains had <97 % 16S rRNA gene sequence similarity with other members of the genus Roseomonas. Strain M3 showed 18±2 and 13±2 % reassociation (based on DNA-DNA hybridization) with R. oryzae KCTC 42542 and Roseomonas cervicalis KACC 11686, respectively. The major cellular fatty acids (>5 %) were identified as C18 : 1ω6c/C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c and C16 : 0 in both strains. Both strains showed diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidylcholine and unidentified glycolipid as major polar lipids. Based on distinct phenotypic, genotypic and phylogenetic differences from the previously described taxa, we propose the classification of strains M3 and M11 as representative of a novel species in the genus Roseomonas, for which the name Roseomonas deserti sp. nov. is suggested. The type strain is M3 (=KEMB 2255-459=JCM 31275).
从科威特受原油污染的沙漠沙中分离出两株深粉色色素沉着的细菌菌株（M3和M11）。两株菌株均为革兰氏阴性菌，形态为小杆状至椭圆形。菌株M3和M11在13-42°C（最适温度为30-35°C）和pH 6.5-9.0（最适pH为7.0-7.5）条件下生长。两株菌株生长均无需额外添加氯化钠。菌株M3和M11的基因组DNA G+C含量分别为69.5和69.0 mol%。两株菌株亲缘关系密切，平均DNA-DNA杂交值为92±1%。两株菌株的16S rRNA基因序列比较表明它们属于玫瑰单胞菌属。菌株M3和M11与米氏玫瑰单胞菌JC288的序列相似性分别为97.3%和97.4%。两株菌株与玫瑰单胞菌属的其他成员的16S rRNA基因序列相似性均<97%。菌株M3与米氏玫瑰单胞菌KCTC 42542和宫颈玫瑰单胞菌KACC 11686的重结合率（基于DNA-DNA杂交）分别为18±2%和13±2%。两株菌株的主要细胞脂肪酸（>5%）均鉴定为C18:1ω6c/C18:1ω7c、C16:1ω6c/C16:1ω7c和C16:0。两株菌株均显示二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和未鉴定的糖脂为主要极性脂质。基于与先前描述的分类单元明显的表型、基因型和系统发育差异，我们提议将菌株M3和M11分类为玫瑰单胞菌属一个新物种的代表，建议将其命名为沙漠玫瑰单胞菌（Roseomonas deserti sp. nov.）。模式菌株为M3（=KEMB 2255-459=JCM 31275）。
