Anderson J V, Christofides N D, Bloom S R
J Endocrinol. 1986 Apr;109(1):9-13. doi: 10.1677/joe.0.1090009.
The response of plasma atrial natriuretic peptide (ANP) concentration to acute intravascular volume expansion was measured in ten male Wistar rats. An infusion of 3 ml polygelene colloidal solution at 37 degrees C over 45 s produced peak venous pressure rises of 1.5 cm water. A highly significant (P less than 0.001) rise of immunoreactive plasma ANP from 24.4 +/- 2.2 (mean +/- S.E.M.) pmol/l to a peak of 70.0 +/- 10.5 pmol/l occurred within 2.5 min. Plasma ANP concentrations had virtually returned to basal levels (32.7 +/- 2.7 pmol/l) 30 min after this acute volume load. A further infusion of 10 ml polygelene colloidal solution in 2 min produced peak venous pressure rises of 10 cm water and caused a dramatic and significant (P less than 0.001) increase of plasma ANP concentration to a peak of 534.8 +/- 38.5 pmol/l, occurring 7.5 min after infusion. The plasma ANP concentration had fallen but remained above basal levels 30 min later (137.2 +/- 26.4 pmol/l). Similar results were obtained using an identical protocol but with whole rat blood instead of polygelene solution as the volume-expanding agent. Gel column chromatography suggested that the majority of the immunoreactive ANP in rat plasma was of similar molecular size to rat alpha-ANP (1-28). These results support the hypothesis that blood volume expansion is a potent stimulus for the release of ANP into plasma.
在十只雄性Wistar大鼠中测量了血浆心钠素（ANP）浓度对急性血管内容量扩张的反应。在45秒内输注37℃的3毫升聚明胶肽胶体溶液，使静脉压峰值升高1.5厘米水柱。免疫反应性血浆ANP从24.4±2.2（平均值±标准误）皮摩尔/升显著升高（P<0.001）至峰值70.0±10.5皮摩尔/升，在2.5分钟内出现。在这种急性容量负荷后30分钟，血浆ANP浓度实际上已恢复到基础水平（32.7±2.7皮摩尔/升）。在2分钟内进一步输注10毫升聚明胶肽胶体溶液，使静脉压峰值升高10厘米水柱，并导致血浆ANP浓度急剧且显著升高（P<0.001）至峰值534.8±38.5皮摩尔/升，在输注后7.5分钟出现。30分钟后血浆ANP浓度下降但仍高于基础水平（137.2±26.4皮摩尔/升）。使用相同方案，但用全鼠血代替聚明胶肽溶液作为容量扩张剂，获得了相似的结果。凝胶柱色谱法表明，大鼠血浆中大多数免疫反应性ANP的分子大小与大鼠α-ANP（1-28）相似。这些结果支持血容量扩张是ANP释放到血浆中的有效刺激这一假说。
