Kato J, Kida O, Higa T, Sasaki A, Kondo K, Miyata A, Kangawa K, Matsuo H, Tanaka K
Life Sci. 1986 Aug 11;39(6):493-7. doi: 10.1016/0024-3205(86)90504-7.
To investigate the releasing mechanisms of atrial natriuretic polypeptide (ANP), identical amounts of 5% glucose solution, isotonic (0.9%) or hypertonic (5%) saline were infused intravenously for 5 min (2 ml/min) in anesthetized rats. At the same time, plasma immunoreactive ANP (ir-ANP) was measured using a direct radioimmunoassay. Plasma ir-ANP increased after infusion of 5% glucose solution (P less than 0.01) and isotonic saline (P less than 0.05), and returned rapidly to the basal levels in the recovery period. Plasma ir-ANP increased to a greater degree in the group infused with hypertonic saline than in the other two groups. The major immunoreactive component of increased ir-ANP was identified as alpha-rat ANP, a 28 amino acid residue, by using reverse phase high-performance liquid chromatography. These results suggest that sodium ions may be a stimulating factor of ANP release as well as volume expansion.
为研究心房利钠多肽（ANP）的释放机制，给麻醉大鼠静脉内输注等量的5%葡萄糖溶液、等渗（0.9%）或高渗（5%）盐水5分钟（2毫升/分钟）。同时，采用直接放射免疫分析法测定血浆免疫反应性ANP（ir-ANP）。输注5%葡萄糖溶液（P<0.01）和等渗盐水（P<0.05）后血浆ir-ANP升高，且在恢复期迅速恢复至基础水平。输注高渗盐水组的血浆ir-ANP升高程度大于其他两组。通过反相高效液相色谱法确定，升高的ir-ANP的主要免疫反应成分是α-大鼠ANP，一种含28个氨基酸残基的肽。这些结果提示，钠离子可能与容量扩张一样，是ANP释放的刺激因素。
