Hu Jianwen, Han Jizhong, Li Haoran, Zhang Xian, Liu Lan Lan, Chen Fei, Zeng Bin
Cells Tissues Organs. 2018;205(1):1-8. doi: 10.1159/000485501. Epub 2018 Feb 1.
Mammalian cells, e.g., CHO, BHK, HEK293, HT-1080, and NS0 cells, represent important manufacturing platforms in bioengineering. They are widely used for the production of recombinant therapeutic proteins, vaccines, anticancer agents, and other clinically relevant drugs. HEK293 (human embryonic kidney 293) cells and their derived cell lines provide an attractive heterologous system for the development of recombinant proteins or adenovirus productions, not least due to their human-like posttranslational modification of protein molecules to provide the desired biological activity. Secondly, they also exhibit high transfection efficiency yielding high-quality recombinant proteins. They are easy to maintain and express with high fidelity membrane proteins, such as ion channels and transporters, and thus are attractive for structural biology and electrophysiology studies. In this article, we review the literature on HEK293 cells regarding their origins but also stress their advancements into the different cell lines engineered and discuss some significant aspects which make them versatile systems for biopharmaceutical manufacturing, drug screening, structural biology research, and electrophysiology applications.
哺乳动物细胞，例如中国仓鼠卵巢细胞（CHO）、幼仓鼠肾细胞（BHK）、人胚肾293细胞（HEK293）、人纤维肉瘤细胞（HT - 1080）和鼠骨髓瘤细胞（NS0），是生物工程中重要的生产平台。它们被广泛用于生产重组治疗性蛋白质、疫苗、抗癌药物和其他临床相关药物。人胚肾293细胞及其衍生细胞系为重组蛋白的开发或腺病毒生产提供了一个有吸引力的异源系统，这尤其是因为它们对蛋白质分子进行类似人类的翻译后修饰以提供所需的生物活性。其次，它们还表现出高转染效率，能产生高质量的重组蛋白。它们易于培养，并且能高保真地表达膜蛋白，如离子通道和转运蛋白，因此对结构生物学和电生理学研究具有吸引力。在本文中，我们回顾了关于人胚肾293细胞起源的文献，同时强调了它们向不同工程细胞系的发展，并讨论了一些使其成为生物制药生产、药物筛选、结构生物学研究和电生理学应用通用系统的重要方面。
