Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, China.
J Diabetes Complications. 2018 Mar;32(3):335-341. doi: 10.1016/j.jdiacomp.2017.12.013. Epub 2017 Dec 29.
To investigate TAp63 expression in patients with type 2 diabetes mellitus (T2DM) and the potential correlations between TAp63 and proinflammatory cytokines production and other clinical parameters.
Peripheral blood mononuclear cells (PBMCs) and plasma were collected from 72 T2DM (cases) and 72 healthy subjects (controls). Fasting blood glucose (FBG), fasting insulin (FIN) and a blood lipid profile were measured. The homeostasis model assessment (HOMA) was used to estimate insulin resistance (IR). Plasma tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 were determined. PBMCs isolated from healthy subjects were cultured with or without 33.3 mmol/l glucose or 0.5 mmol/l palmitic acid (PA) for 6 h, 24 h, 48 h, and 72 h. The expression of TAp63 at mRNA and protein levels in PBMCs was analyzed using real-time qRT-PCR and western blots, respectively.
TAp63 expression was significantly lower in T2DM patients compared with that of the controls. In addition, TAp63 expression showed a negative correlation with FBG, FIN, HbA1c, HOMA-IR, FFAs, TNF-α, and IL-6 levels. Treatment with 33.3 mmol/l glucose or 0.5 mmol/l PA increased TAp63 expression in the cultured PBMCs.
TAp63 level may be correlated with chronic inflammatory state and perturbed glucose and lipid metabolism in T2DM.
研究 TAp63 在 2 型糖尿病（T2DM）患者中的表达情况，以及 TAp63 与促炎细胞因子产生和其他临床参数之间的潜在相关性。
收集 72 例 T2DM（病例）和 72 例健康受试者（对照）的外周血单核细胞（PBMC）和血浆。测定空腹血糖（FBG）、空腹胰岛素（FIN）和血脂谱。采用稳态模型评估（HOMA）估计胰岛素抵抗（IR）。测定血浆肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）-6。分离健康受试者的 PBMC，分别在 33.3 mmol/L 葡萄糖或 0.5 mmol/L 棕榈酸（PA）作用 6 h、24 h、48 h 和 72 h 后培养。采用实时 qRT-PCR 和 Western blot 分别分析 PBMC 中 TAp63 的 mRNA 和蛋白表达水平。
与对照组相比，T2DM 患者的 TAp63 表达明显降低。此外，TAp63 表达与 FBG、FIN、HbA1c、HOMA-IR、FFAs、TNF-α和 IL-6 水平呈负相关。用 33.3 mmol/L 葡萄糖或 0.5 mmol/L PA 处理可增加培养的 PBMC 中的 TAp63 表达。
TAp63 水平可能与 T2DM 患者的慢性炎症状态以及葡萄糖和脂质代谢紊乱有关。
