Xue J, Zhao H, Shang G, Zou R, Dai Z, Zhou D, Huang Q, Xu Y
Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Exp Clin Endocrinol Diabetes. 2013 Jan;121(1):37-42. doi: 10.1055/s-0032-1323683. Epub 2012 Sep 6.
To evaluate the expression level of RIP140 (receptor interaction protein 140) and its correlation with inflammatory cytokine production and free fatty acids (FFAs) in type 2 diabetes.
Plasma and peripheral blood mononuclear cells (PBMCs) were collected from 24 diabetic and 30 healthy individuals. The levels of FFAs, TC, TG, HDL-C, LDL-C, FIN, and FBG were measured. The insulin resistance index was calculated using the homeostasis model assessment (HOMA). Additionally, PBMCs from control group were cultured alone or with 500 μmol/L palmitic acid (PA). Levels of RIP140 TNF-α, and IL-6 in PBMCs were analyzed using real-time RT-PCR, Western blots and ELISA. The relationship between RIP140 and other variables was performed using SPSS 11.5 software.
TG, LDL-C, FIN, FBG, HOMA, and HDL-C were significantly different between diabetic patients and the control group. Levels of RIP140, TNF-α, and IL-6 were higher in the diabetic group compared to control. RIP140 expression was positively correlated with FFAs, HDL-c, TNF-α, IL-6, FIN, FBG, and HOMA. Finally, 500 μmol/L PA treatment increased RIP140 expression and the secretion of inflammatory cytokines in cultured control PBMCs.
Increased RIP140 level may be closely associated with inflammation and disorder of lipid and glucose metabolism in diabetic patients.
评估2型糖尿病中RIP140（受体相互作用蛋白140）的表达水平及其与炎性细胞因子产生和游离脂肪酸（FFA）的相关性。
收集24例糖尿病患者和30例健康个体的血浆及外周血单个核细胞（PBMC）。检测FFA、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、空腹胰岛素（FIN）和空腹血糖（FBG）水平。采用稳态模型评估（HOMA）计算胰岛素抵抗指数。此外，将对照组的PBMC单独培养或与500μmol/L棕榈酸（PA）一起培养。使用实时逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法和酶联免疫吸附测定（ELISA）分析PBMC中RIP140、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平。使用SPSS 11.5软件分析RIP140与其他变量之间的关系。
糖尿病患者与对照组之间的TG、LDL-C、FIN、FBG、HOMA和HDL-C存在显著差异。糖尿病组中RIP140、TNF-α和IL-6的水平高于对照组。RIP140表达与FFA、HDL-c、TNF-α、IL-6、FIN、FBG和HOMA呈正相关。最后，500μmol/L PA处理增加了培养的对照PBMC中RIP140的表达和炎性细胞因子的分泌。
RIP140水平升高可能与糖尿病患者的炎症及脂质和葡萄糖代谢紊乱密切相关。
