Huang Qi, Xue Junli, Zou Runmei, Cai Li, Chen Jing, Sun Li, Dai Zhe, Yang Fan, Xu Yancheng
Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
Exp Ther Med. 2014 Nov;8(5):1648-1654. doi: 10.3892/etm.2014.1958. Epub 2014 Sep 11.
Type 2 diabetes (T2D) is a common disorder characterized by chronic low-grade inflammation. In the present study, the expression levels of nuclear receptor subfamily 4 group A member 1 (NR4A1) and the correlation with inflammatory cytokine production and free fatty acids (FFAs) in patients with T2D and healthy participants were investigated. NR4A1 expression levels in peripheral blood mononuclear cells (PBMCs) from patients with T2D (n=30) and healthy controls (n=34) were analyzed. In addition, the levels of fasting blood glucose (FBG), fasting plasma insulin (FIN), FFAs, total cholesterol (TC), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) were analyzed, and the homeostasis model assessment (HOMA) was used to estimate the insulin resistance (IR). Additionally, PBMCs from healthy subjects were cultured with or without 250 μM palmitic acid (PA). Levels of NR4A1, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the PBMCs were also analyzed. The basal expression levels of NR4A1, TNF-α and IL-6 were higher in the T2D patients when compared with the controls. In addition, the levels of FFAs, TG and LDL-C, as well as the HOMA-IR, were higher in T2D patients. Furthermore, NR4A1 expression was demonstrated to positively correlate with the HOMA-IR and the levels of FFAs, TNF-α, IL-6, FIN and FBG. Furthermore, 250 μM PA stimulation was shown to increase NR4A1 expression and the secretion of inflammatory cytokines in the cultured PBMCs. Therefore, increased NR4A1 expression levels are correlated with a chronic low-grade inflammatory state and the disorder of lipid metabolism in patients with T2D.
2型糖尿病（T2D）是一种以慢性低度炎症为特征的常见疾病。在本研究中，调查了2型糖尿病患者和健康参与者中核受体亚家族4 A组成员1（NR4A1）的表达水平及其与炎性细胞因子产生和游离脂肪酸（FFA）的相关性。分析了30例2型糖尿病患者和34例健康对照者外周血单个核细胞（PBMC）中NR4A1的表达水平。此外，分析了空腹血糖（FBG）、空腹血浆胰岛素（FIN）、FFA、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）水平，并采用稳态模型评估（HOMA）来评估胰岛素抵抗（IR）。此外，将健康受试者的PBMC在有或无250μM棕榈酸（PA）的条件下进行培养。还分析了PBMC中NR4A1、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平。与对照组相比，2型糖尿病患者中NR4A1、TNF-α和IL-6的基础表达水平更高。此外，2型糖尿病患者的FFA、TG和LDL-C水平以及HOMA-IR更高。此外，NR4A1表达与HOMA-IR以及FFA、TNF-α、IL-6、FIN和FBG水平呈正相关。此外，250μM PA刺激可增加培养的PBMC中NR4A1的表达和炎性细胞因子的分泌。因此，NR4A1表达水平升高与2型糖尿病患者的慢性低度炎症状态和脂质代谢紊乱相关。
