BAM Federal Institute for Materials Research and Testing, Department of Analytical Chemistry; Reference Materials, Richard-Willstätter-Str. 11, 12489 Berlin, Germany; Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
BAM Federal Institute for Materials Research and Testing, Department of Analytical Chemistry; Reference Materials, Richard-Willstätter-Str. 11, 12489 Berlin, Germany; Humboldt-Universität zu Berlin, Department of Chemistry, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
J Chromatogr A. 2018 Feb 23;1538:112-116. doi: 10.1016/j.chroma.2018.01.037.
A frequently studied environmental contaminant is the active substance diclofenac, which is removed insufficiently in sewage treatment plants. Since its inclusion in the watch list of the EU Water Framework Directive, the concentrations in surface waters will be determined throughout Europe. For this, still, more precise analytical methods are needed. As a reference, HPLC-MS is frequently employed. One of the major metabolites is 4'-hydroxydiclofenac (4'-OH-DCF). Also, diclofenac lactam is important for assessing degradation and transformation. Aceclofenac (ACF), the glycolic acid ester of diclofenac is used as a drug, too, and could potentially be cleaved to yield diclofenac again. In various sewage treatment plant influent samples, diclofenac, 4'-OH-DCF, DCF lactam and ACF could be determined with detection limits of 3 μg/L, 0.2 μg/L, 0.17 μg/L and 10 ng/L, respectively.
一种经常被研究的环境污染物是活性物质双氯芬酸，它在污水处理厂中去除得不够充分。自从被列入欧盟水框架指令的观察名单以来，整个欧洲都将测定地表水中的浓度。为此，仍需要更精确的分析方法。高效液相色谱-质谱法（HPLC-MS）经常被用作参考。主要代谢物之一是 4'-羟基双氯芬酸（4'-OH-DCF）。此外，双氯芬酸内酰胺对于评估降解和转化也很重要。醋氯芬酸（ACF）是双氯芬酸的甘醇酸酯，也被用作药物，并且有可能再次被裂解生成双氯芬酸。在各种污水处理厂进水样本中，可以测定到双氯芬酸、4'-OH-DCF、双氯芬酸内酰胺和 ACF，检测限分别为 3μg/L、0.2μg/L、0.17μg/L 和 10ng/L。
